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11040596001

Roche

Interferon-γ, human (hIFN-γ)

from human, 95% (SDS-PAGE), solution, >2 x 10^7 U/mg

Synonym(s):

SYBR, qPCR

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About This Item

UNSPSC Code:
12352207

biological source

human

Quality Level

recombinant

expressed in E. coli

description

recombinant (E. coli)

Assay

95% (SDS-PAGE)

form

solution

potency

<0.05 ng/mL EC50

specific activity

>2 x 10^7 U/mg

mol wt

16,700 Da

packaging

pkg of 100,000 U (5 μg, 1 ml)

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

impurities

<0.1 EU/μgtested (LAL test)

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IFNG(3458)

General description

IFN-γ is produced by T-lymphocytes stimulated by antigen or by T-cell mitogens. Under nondenaturating conditions its molecular weight values range from 32,000 to 73,000 indicating that recombinant human IFN-γ exists as a dimer or higher oligomers.

Specificity

Human IFN-γ is effective on human cells, but not on mouse or rat cells.

Application

Interferon-γ, human (hIFN-γ) has been used to stimulate mesenchymal stem cells (MSCs). It has also been used as a supplement Dulbecco′s modified Eagle′s medium/Ham′s medium for the cultivation of human colorectal carcinoma cell line T84 cells.
Interferon-γ, human (hIFN-γ), can be used to investigate IFN-γ activities in human cell systems.

Biochem/physiol Actions

A broad range of biological activities has been attributed to IFN-γ (e.g., the establishment of the antiviral state, immunoregulatory functions, antiproliferative effects and inhibition of cell growth). The anti-proliferative effects of IFN-γ are superior to those of either IFN-α or IFN-β. Growth inhibition is dependent on cell type, dose, and length of exposure. One of IFN-γ′s primary functions might be as an immunoregulatory agent: IFN-γ induces MHC antigens on many cells, Fc-receptors on monocytes and macrophages, IL-2 receptors on T-cells, enhances activity of macrophages, polymorphonuclear leukocytes, T-lymphocytes, and NK-cells (MAF), and is also involved in the regulation of B-cells.
Interferon-γ (IFN-γ) is an essential component of acquired immunity. A broad range of biological activities has been attributed to IFN-γ (e.g., the establishment of the antiviral state, immunoregulatory functions, antiproliferative effects and inhibition of cell growth). The anti-proliferative effects of IFN-γ are superior to those of either IFN-α or IFN-β. Growth inhibition is dependent on cell type, dose, and length of exposure. One of IFN-γ′s primary functions might be as an immunoregulatory agent: IFN-γ induces MHC antigens on many cells, Fc-receptors on monocytes and macrophages, IL-2 receptors on T-cells, enhances activity of macrophages, polymorphonuclear leukocytes, T-lymphocytes, and NK-cells (MAF), and is also involved in the regulation of B-cells.

Quality

The raw material used for this preparation was tested for HBs antigen and for the presence of antibodies to HIV-1, HIV-2, and HCV found to be negative.
Endotoxin level: <0.1EU/μg (LAL-test), <10EU/ml (LAL-test)
Note: 1EU corresponds to 0.1ng

Sequence

Chain Length 143 AA
The primary structure of recombinant human IFN-γ (143 amino acids) is identical to that of natural human IFN-γ (146 amino acids), however, recombinant IFN-γ has three amino acids less and is not glycosylated. Glycosylation is not essential for biological activity.

Unit Definition

EC50 definition: The amount of hIFN-γ that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN-γ reference standard (Gg 23– 901-530) (WISH cells-EMC virus/cytopathic effect) (1 unit equals ≤0.05 ng/ml).

Physical form

100,000U/ml or 1,000,000U/ml of Human IFN-γ in 0.1M phosphate buffer saline (PBS) (pH 7.0), 2.5% sucrose (w/v), and 2.5% human serum albumin (HSA) (w/v), filtered through a 0.2μm pore size membrane.

Preparation Note

Working solution: Solvent is recommended in double-distilled water.
Storage conditions (working solution): Stability in culture: Activity is present for at least 18 hours in culture medium.

Other Notes

EC 50 : <0.05ng/ml (hIFN-γ , NIH, reference standard, Gg 23–901-530)
For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available


Certificates of Analysis (COA)

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Anti-proliferative effect of IFN-gamma in immune regulation. II. IFN-gamma inhibits the proliferation of murine bone marrow cells stimulated with IL-3, IL-4, or granulocyte-macrophage colony-stimulating factor
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Impaired immune function contributes to the development of chronic obstructive pulmonary disease (COPD). Disease progression is further exacerbated by pathogen infections due to impaired immune responses. Elimination of infected cells is achieved by cytotoxic CD8(+)  T cells that are activated

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