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N27-A Rat Dopaminergic Neural Cell Line

Rat

Synonym(s):

N27-A Neural Cells, Rat Dopaminergic Cells

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81

product name

N27-A Rat Dopaminergic Neural Cell Line, N27-A rat dopaminergic neural cell line is a suitable model for Parkinson’s disease research.

biological source

rat

technique(s)

cell culture | mammalian: suitable

General description

N27-A is a clonal derivative of N27 characterized by consistently high expression of the dopaminergic markers TH, dopamine transporter (DAT), and Tuj1 . N27-A cells express the dopaminergic neuron transcription factors Nurr1, En1, FoxA2, and Pitx3 as well as the monoamine transporter VMAT2, but do not express dopamine-beta-hydroxylase (DbH), the enzyme that converts dopamine to norepinephrine. N27-A cells exhibit higher sensitivity to neurotoxins as compared to parental N27 cells, and release dopamine under both basal and depolarization conditions. These features make the N27-A cell line an improved model for Parkinson′s disease research.
Source:
The N27-A cell line is a clonal derivative of the parental N27 cell line selected for high expression of dopaminergic neural markers. The parental cell line was originally isolated from dopaminergic neurons from an embryonic day 14 rat mesencephalon and immortalized with SV40 large T antigen .
Parkinson’s disease is characterized by the death of dopaminergic neurons in the substantia nigra of the brain . In vitro models are critical for understanding the molecular pathology of Parkinson’s disease. The N27 immortalized rat dopaminergic cell line has been widely utilized in Parkinson’s research for over 20 years. However long-term passaging has led the N27 cell line to become a mixture of cell types with widely variable expression of dopaminergic neural markers such as tyrosine hydroxylase (TH).

Cell Line Description

Neural Lineage Cells

Application

N27-A rat dopaminergic neural cell line is a suitable model for Parkinson’s disease research.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins

Neurodegenerative Diseases
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Quality

• Each vial contains ≥ ≥1X10⁶ cells/vial.
• Cells are tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of rat origin and negative for inter-species contamination from mouse, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Disclaimer

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lu Gao et al.
PloS one, 11(8), e0160847-e0160847 (2016-08-12)
Parkinson's disease is characterized by the death of dopaminergic neurons in the substantia nigra. To understand the molecular mechanisms of the disease, an in vitro model is important. In the 1990s, we used the SV40 large T antigen to immortalize
David T Dexter et al.
Free radical biology & medicine, 62, 132-144 (2013-02-06)
Parkinson disease (PD) is a complex neurodegenerative disorder with both motor and nonmotor symptoms owing to a spreading process of neuronal loss in the brain. At present, only symptomatic treatment exists and nothing can be done to halt the degenerative
E D Clarkson et al.
Proceedings of the National Academy of Sciences of the United States of America, 95(3), 1265-1270 (1998-03-14)
The replacement of dopamine (DA) by DA neuron transplants in the treatment of advanced Parkinson disease (PD) is a rational approach. Because of limitations associated with fetal tissue transplants, a clone (1RB3AN27) of simian virus 40 large tumor antigen (LTa)

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