The RTHYMAG-30K kit utilizes a competitive assay for T3 and T4 and a sandwich assay for TSH. The T3 and T4 assay requires HRP Conjugate. In this assay, the bead is conjugated with the capture antibody, the standard cocktail contains T3 and T4 analytes that are unlabeled, and the LRTH-HRP includes T3 and T4 conjugated with HRP. During the assay, the unlabeled (standard) and labeled LRTH-HRP compete for the capture antibody on the beads. A detection antibody containing Anti-HRP-Biotinylated is then added. In the absence of the standard, the maximum amount of the labeled T3-T4 (LRTH-HRP) will bind to the bead and can be detected by the anti-HRP-Biotin – SAPE complex. As increasing amounts of standard are added, it competes out the labeled analyte, resulting in a decrease in the signal.
RTHYMAG-30K
MILLIPLEX® Rat Thyroid Magnetic Bead Panel - Endocrine Multiplex Assay
The analytes available for this multiplex kit are: TSH, T3, T4.
About This Item
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Quality Level
species reactivity
rat
manufacturer/tradename
Milliplex®
assay range
sensitivity: 2255 pg/mL
(T4)
sensitivity: 284 pg/mL
(T3)
sensitivity: 38 pg/mL
(TSH)
standard curve range: 41-10,000 pg/mL
(TSH & T3)
standard curve range: 823-200,00 pg/mL
(T4)
inter-assay cv: <15%
intra-assay cv: <10%
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
General description
MILLIPLEX® Rat Thyroid Hormone Panel is a 3-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue culture samples: T3 (competitive assay), T4 (competitive assay), and TSH. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
Specificity
There was no or negligible cross-reactivity between the analytes within the panel.
Application
- Analytes: T3, T4, TSH
- NOTE: T3 and T4 are competitive immunoassays
- Recommended Sample Type: Rat serum, plasma, cell/tissue culture supernatants or lysates
- Recommended Sample Dilution: 25 μL per well 1:6 diluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
- Assay Run Time: Overnight (16-18 hours) at 2-8°C
- Research Category: Endocrine
- Research Category: Metabolism
Features and Benefits
Other Notes
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
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Related Content
Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
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Why does the Rat Thyroid kit include an HRP conjugate?
1 answer-
Helpful?
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Could you provide information on whether the RTHYMAG-30K assay is capable of measuring free T4?
1 answer-
The test measures both total T3 and T4 levels.
Helpful?
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Can Milliplex Rat Thyroid Panel (RTHYMAG-30K) be used for analyzing mouse samples?
1 answer-
The antibodies are cross-reactive to mouse but have not been full validated with mouse samples. The sample dilution and Serum Matrix have not been optimized. The references provided below have used the RTHYMAG kit with mouse samples and may be helpful.
Liu YY, Jiang J, Ke S, Milanesi A, Abe K, Gastelum G, Li J, Brent GA.
Thyroid hormone receptor alpha sumoylation modulates white adipose tissue stores.
Sci Rep. 2021; 11(1):24105.
Fonseca TL, Werneck-De-Castro JP, Castillo M, Bocco BM, Fernandes GW, McAninch EA, Ignacio DL, Moises CC, Ferreira A, Gereben B, Bianco AC.
Tissue-Specific Inactivation of Type II Deiodinase Reveals Multi-Level Control of Fatty Acid Oxidation by Thyroid Hormone in the Mouse.
Diabetes. 2014; 63(5):1594-604
Vella KR, Ramadoss P, Costa-E-Sousa RH, Astapova I, Ye FD, Holtz KA, Harris JC, Hollenberg AN.
Thyroid Hormone Signaling in vivo Requires a Balance Between Coactivators and Corepressors.
Mol Cell Biol. 2014; 34(9):1564-75
Gill JC, Navarro VM, Kwong C, Noel SD, Martin C, Xu S, Clifton DK, Carroll RS, Steiner RA, Kaiser UB.
Increased neurokinin B (Tac2) expression in the mouse arcuate nucleus is an early marker of pubertal onset with differential sensitivity to sex steroid-negative feedback than Kiss1.
Endocrinology. 2012; 153(10):4883-93.
Ueta CB, Olivares EL, Bianco AC.
Responsiveness to thyroid hormone and to ambient temperature underlies differences between brown adipose tissue and skeletal muscle thermogenesis in a mouse model of diet-induced obesity.
Endocrinology. 2011 Sep;152(9):3571-81.
Hall JA, Ribich S, Christoffolete MA, Simovic G, Correa-Medina M, Patti ME, Bianco AC.
Absence of thyroid hormone activation during development underlies a permanent defect in adaptive thermogenesis.
Endocrinology. 2010; 151(9):4573-82Helpful?
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