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RTHYMAG-30K

Millipore

MILLIPLEX® Rat Thyroid Magnetic Bead Panel - Endocrine Multiplex Assay

The analytes available for this multiplex kit are: TSH, T3, T4.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
To order a Milliplex® kit, please search for your analyte of interest.
Design And Price Your Kit

Quality Level

200

species reactivity

rat

manufacturer/tradename

Milliplex®

assay range

sensitivity: 2255 pg/mL
(T4)
sensitivity: 284 pg/mL
(T3)
sensitivity: 38 pg/mL
(TSH)
standard curve range: 41-10,000 pg/mL
(TSH & T3)
standard curve range: 823-200,00 pg/mL
(T4)
inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

The synthesis and release of Triiodothyronine (T3) and thyroxine (T4) are regulated by Thyroid-Stimulating Hormone (TSH), which is secreted by the anterior pituitary gland. These thyroid hormones play critical roles in the regulation of energy balance, metabolism of carbohydrates, fatty acids and proteins. Thyroid hormones are critical to the development of the central nervous and skeletal systems. The simultaneous monitoring of all three hormones can be of great value when researching disease models and therapeutic response.

MILLIPLEX® Rat Thyroid Hormone Panel is a 3-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue culture samples: T3 (competitive assay), T4 (competitive assay), and TSH. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Endocrine

Specificity

Cross Reactivty
There was no or negligible cross-reactivity between the analytes within the panel.

Application

  • Analytes: T3, T4, TSH
  • NOTE: T3 and T4 are competitive immunoassays
  • Recommended Sample Type: Rat serum, plasma, cell/tissue culture supernatants or lysates
  • Recommended Sample Dilution: 25 μL per well 1:6 diluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Endocrine
  • Research Category: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The prospective protective effect of selenium nanoparticles against chromium-induced oxidative and cellular damage in rat thyroid.
Hassanin, KM; Abd El-Kawi, SH; Hashem, KS
International journal of nanomedicine null
Giulia Di Dalmazi et al.
Journal of immunology (Baltimore, Md. : 1950), 202(5), 1350-1362 (2019-01-25)
MYMD-1 is a synthetic derivative of tobacco alkaloids, compounds that possess immunoregulatory properties and have been linked to the epidemiological observation that smoking reduces the odds of developing thyroid Abs and hypothyroidism. To assess the effect and mechanism(s) of the
Rauf Latif et al.
Frontiers in endocrinology, 12, 706101-706101 (2021-07-20)
The synergistic activation of transcription factors can lead to thyroid progenitor cell speciation. We have previously shown in vitro that mouse or human stem cells, expressing the transcription factors NKx2-1 and Pax8, can differentiate into thyroid neo-follicular structures (TFS). We
Sangho Yu et al.
eLife, 7 (2018-05-16)
The adipokine leptin acts on the brain to regulate energy balance but specific functions in many brain areas remain poorly understood. Among these, the preoptic area (POA) is well known to regulate core body temperature by controlling brown fat thermogenesis
Anthony R P Verkerke et al.
Nature metabolism, 1(9), 876-885 (2020-05-15)
The biophysical environment of membrane phospholipids affects structure, function, and stability of membrane-bound proteins.1,2 Obesity can disrupt membrane lipids, and in particular, alter the activity of sarco/endoplasmic reticulum (ER/SR) Ca2+-ATPase (SERCA) to affect cellular metabolism.3-5 Recent evidence suggests that transport
View All Related Papers

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Multiplexing Metabolic Syndrome Biomarkers | MILLIPLEX® Assays

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

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Questions

1–3 of 3 Questions  

  1. Why does the Rat Thyroid kit include an HRP conjugate?

    1 answer

    1. The RTHYMAG-30K kit utilizes a competitive assay for T3 and T4 and a sandwich assay for TSH. The T3 and T4 assay requires HRP Conjugate. In this assay, the bead is conjugated with the capture antibody, the standard cocktail contains T3 and T4 analytes that are unlabeled, and the LRTH-HRP includes T3 and T4 conjugated with HRP. During the assay, the unlabeled (standard) and labeled LRTH-HRP compete for the capture antibody on the beads. A detection antibody containing Anti-HRP-Biotinylated is then added. In the absence of the standard, the maximum amount of the labeled T3-T4 (LRTH-HRP) will bind to the bead and can be detected by the anti-HRP-Biotin – SAPE complex. As increasing amounts of standard are added, it competes out the labeled analyte, resulting in a decrease in the signal.

      Helpful?

  2. Could you provide information on whether the RTHYMAG-30K assay is capable of measuring free T4?

    1 answer

    1. The test measures both total T3 and T4 levels.

      Helpful?

  3. Can Milliplex Rat Thyroid Panel (RTHYMAG-30K) be used for analyzing mouse samples?

    1 answer

    1. The antibodies are cross-reactive to mouse but have not been full validated with mouse samples.  The sample dilution and Serum Matrix have not been optimized. The references provided below have used the RTHYMAG kit with mouse samples and may be helpful.
      Liu YY, Jiang J, Ke S, Milanesi A, Abe K, Gastelum G, Li J, Brent GA.
      Thyroid hormone receptor alpha sumoylation modulates white adipose tissue stores.
      Sci Rep. 2021; 11(1):24105.
      Fonseca TL, Werneck-De-Castro JP, Castillo M, Bocco BM, Fernandes GW, McAninch EA, Ignacio DL, Moises CC, Ferreira A, Gereben B, Bianco AC.
      Tissue-Specific Inactivation of Type II Deiodinase Reveals Multi-Level Control of Fatty Acid Oxidation by Thyroid Hormone in the Mouse.
      Diabetes. 2014; 63(5):1594-604
      Vella KR, Ramadoss P, Costa-E-Sousa RH, Astapova I, Ye FD, Holtz KA, Harris JC, Hollenberg AN.
      Thyroid Hormone Signaling in vivo Requires a Balance Between Coactivators and Corepressors.
      Mol Cell Biol. 2014; 34(9):1564-75
      Gill JC, Navarro VM, Kwong C, Noel SD, Martin C, Xu S, Clifton DK, Carroll RS, Steiner RA, Kaiser UB.
      Increased neurokinin B (Tac2) expression in the mouse arcuate nucleus is an early marker of pubertal onset with differential sensitivity to sex steroid-negative feedback than Kiss1.
      Endocrinology. 2012; 153(10):4883-93.
      Ueta CB, Olivares EL, Bianco AC.
      Responsiveness to thyroid hormone and to ambient temperature underlies differences between brown adipose tissue and skeletal muscle thermogenesis in a mouse model of diet-induced obesity.
      Endocrinology. 2011 Sep;152(9):3571-81.
      Hall JA, Ribich S, Christoffolete MA, Simovic G, Correa-Medina M, Patti ME, Bianco AC.
      Absence of thyroid hormone activation during development underlies a permanent defect in adaptive thermogenesis.
      Endocrinology. 2010; 151(9):4573-82

      Helpful?

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