MHA00FCR2
m-FC Broth without Rosolic Acid
Synonym(s):
m-FC Broth
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About This Item
UNSPSC Code:
41106225
eCl@ss:
32160801
NACRES:
NA.74
Recommended Products
pH
7.4±0.2 (25.0 °C)
Quality Level
compatibility
for use with MF-method
for use with Microfil®
for use with MicropreSure®
for use with Milliflex®
General description
m-FC Broth without Rosolic Acid is recommended for the detection and enumeration of faecal coliforms using the membrane filter technique.{21} Eliminating rosolic acid from M-FC medium improves the MFC procedure by allowing higher fecal coliform colony recoveries with greater ease. Eliminating rosolic acid from M-FC medium, fecal coliform colonies form an intense royal blue colony on membrane filters. The vivid blue color of the colonies makes counting of fecal coliform colonies easier than when rosolic acid was used because of the sharper contrast between fecal and non fecal colonies. Fecal coliforms appear blue, other colonies appear gray to cream coloured
Application
m-FC Broth without rosolic acid is used to grow faecal coliforms in water and effluent samples thus aiding in Environmental monitoring.{22}
Packaging
2 mL plastic ampoules
Specifications
Incubation Temperature: 44.5 °C ± 0.2
Incubation Time: 24 hrs ± 2
Standard Methods for the Examination of Water and Wastewater
Culture Medium
m-FC w/o Rosolic Acid
Analysis Note
Fecal coliforms appear blue, other colonies appear gray to cream colored
QC organisms
E. coli (ATCC 25922)
Mixed culture (E. coli, P. aeruginosa and P. vulgaris)
E. coli (ATCC 25922)
Mixed culture (E. coli, P. aeruginosa and P. vulgaris)
Target Microorganisms: Fecal coliforms
Legal Information
MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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American Public Health Association
Standard Methods for Examination of Water and Wastewater, 2(2), 208-214 (2005)
Fecal-coliform-organism medium for the membrane filter technique
Geldreich EE et al.
J. Amer. Water Works Assoc., 57(2), 208-214 (1965)
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