218832

Caspase-3/7 Inhibitor II

The Caspase-3/7 Inhibitor II, also referenced under CAS 775289-20-8, controls the biological activity of Caspase-3/7. This small molecule/inhibitor is primarily used for Cancer applications.

• Synonym(s): Caspase-3/7 Inhibitor II, Ac-DNLD-CHO
• Empirical Formula (Hill Notation): C₂₀H₃₁N₅O₁₀
• CAS Number: 775289-20-8
• Molecular Weight: 501.49
• MDL number: MFCD07368597
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: white
• solubility: water: 1 mg/mL; DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: N([C@@H](CC(C)C)C(=O)N[C@@H](CC(=O)O)C=O)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)C)CC(=O)O)CC(=O)N
• InChI key: XZGUQURUQBIHMG-XUXIUFHCSA-N

Description

General description

A tetrapeptidyl aldehyde that acts as a potent, reversible and active site binding inhibitor of caspases-3 and -7 (IC₅₀ = 3.2 nM and 22.6 nM, respectively) and displays ~100-fold greater selectivity over caspases-8 and -9 (IC₅₀ = 577.6 nM and 364.7 nM, respectively). Offers protection against Camptothecin (Cat. No. 208925), and anti-Fas-mediated apoptosis in Jurkat T cells.

A tetrapeptidyl aldehyde that acts as a potent, reversible and active site binding inhibitor of caspases-3 and -7 (IC₅₀ = 3.2 nM and 22.6 nM, respectively) and displays ~100-fold greater selectivity over caspases-8 and -9 (IC₅₀ = 577.6 nM and 364.7 nM, respectively). Offers protection against Camptothecin (Cat. No. 208925), and anti-Fas-mediated apoptosis in Jurkat T cells.

Note: aldehyde-based inhibitors are less cell-permeable than FMK-based inhibitors, so higher concentrations may be required for cell-based inhibition studies (e.g. 100 µM).

Biochem/physiol Actions

Cell permeable: no

Primary Target
caspase-3, caspase-7

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: 3.2 nM and 22.6 nM, against caspases-3 and -7, respectively

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Asp-Asn-Leu-Asp-CHO

Analysis Note

Single main spot with additional trace spot by TLC

Other Notes

Yoshimori, A., et al. 2004. BMC Pharmacol.4, 7.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable