1.11474

1,4-Dithiothreitol

for biochemistry

• Synonym(s): 1,4-Dithiothreitol, DTT, Cleland’s reagent
• Linear Formula: (C₂H₃OHSH)₂
• CAS Number: 3483-12-3
• Molecular Weight: 154.25
• MDL number: MFCD00004877
• UNSPSC Code: 12352104
• EC Index Number: 222-468-7
• NACRES: NB.21

Properties

• grade: for biochemistry
• Quality Level: 200
• Assay: ≥99% (iodometric)
• form: solid
• potency: 400 mg/kg LD₅₀, oral (Rat)
• reaction suitability: reagent type: reductant
• pH: 5.1 (20 °C, 10 g/L in H₂O)
• mp: 40-43 °C
• transition temp: flash point >110 °C
• bulk density: 300 kg/m³
• storage temp.: 2-8°C
• SMILES string: SC[C@H](O)[C@@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
• InChI key: VHJLVAABSRFDPM-IMJSIDKUSA-N

Description

Application

• Drug delivery using reduction-responsive hydrogel based on carboxyethyl-succinoglycan with highly improved rheological, antibacterial, and antioxidant properties.: This study explores the application of 1,4-Dithiothreitol in developing reduction-responsive hydrogels, highlighting significant enhancements in rheological, antibacterial, and antioxidant characteristics, suitable for drug delivery systems (Kim et al., 2024).


• Preparation and properties of hydrogel photonic crystals assembled by biodegradable nanogels.: Research discusses the integration of 1,4-Dithiothreitol in the assembly of hydrogel photonic crystals using biodegradable nanogels, emphasizing their potential in creating novel photonic materials (Zhang et al., 2024).


• Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis.: The paper highlights the use of 1,4-Dithiothreitol in optimizing RNA isolation techniques from bovine sperm, which enhances sperm head lysis, crucial for reproductive biology studies (Tiwari et al., 2024).


• Preparation of a Convertible Spacer Containing a Disulfide Group for Versatile Functionalization of Oligonucleotides.: Discusses the synthesis of a convertible spacer containing a disulfide group using 1,4-Dithiothreitol, enabling versatile functionalization of oligonucleotides for therapeutic and diagnostic applications (Pontarelli et al., 2023).

Analysis Note

Assay (iodometric): ≥ 99 %
Identity (IR-spectrum): passes test
Melting point: 40 - 43 °C
Heavy metals (as Pb): ≤ 10 ppm
Fe (Iron): ≤ 5 ppm

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H302 - H315 - H318
• Precautionary Statements: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable