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T7782

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)–TRITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

TRITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:64
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Immunoglobulin G (IgG)antibody is found in four subclasses IgG1, IgG2, IgG3 and IgG4. It has a monomeric structure. IgG is secreted by B cells and is found in blood and extracellular fluids.. Anti-Mouse IgG (Fab specific) is conjugated to tetramethylrhodamine isothiocyanate (TRITC) and then purified by gel filtration to remove free TRITC.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Conjugation of TRITC to Mouse IgG (Fab specific) antibody enables the use in immunofluorescent techniques.

Biochem/physiol Actions

IgG antibody subtype provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) antibody participates in complement fixation and opsonization.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with bovine, horse or human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sh Hejazi et al.
Iranian journal of parasitology, 7(2), 53-60 (2012-10-31)
Cutaneous leishmaniasis is a neglected parasitic disease, which imposes massive human distress and financial costs to the endemic countries. Better understanding of host immune response to the parasite leads to helpful strategies for disease control. Interleukin (IL)-10 and transforming growth
Sh Hejazi et al.
Iranian journal of parasitology, 7(3), 16-23 (2012-10-31)
Cutaneous leishmaniasis is a neglected parasitic disease, which imposes massive human distress and financial costs to the endemic countries. Better understanding of host immune response to the parasite leads to helpful strategies for disease control. Interleukin (IL)-10 and transforming growth
Shervin G Hoseini et al.
Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, 17(6), 513-518 (2013-04-30)
Cutaneous leishmaniasis (CL) is a public health problem in several endemic countries. Recent studies on mouse model and also a few clinical experiments showed that the type of immune response generated at the site of infection and especially balance between
M Voldstedlund et al.
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 105(7), 537-545 (1997-07-01)
Most cells express facilitative glucose transporters. Four isoforms (GLUT1-4) transporting D-glucose across the plasma membrane show a specific tissue distribution, which is the basis for tissue-specific patterns in glucose metabolism. GLUT1 is expressed at high levels in tissue barriers such
Hua Jiang et al.
Molecular oncology, 8(2), 297-310 (2014-01-07)
Reprogramming of NK cells with a chimeric antigen receptor (CAR) proved an effective strategy to increase NK cell reactivity and recognition specificity toward tumor cells. To enhance the cytotoxicity of NK cells against CD138-positive multiple myeloma (MM) cells, we generated

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