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P9740

PHOS-Select™ Iron Affinity Gel

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Form:
suspension
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form

suspension

Quality Segment

200

shelf life

2 yr at −20‑0 °C

pH

~5

capacity

≥2 μmol/mL, gel (phosphopeptide)(following a 30 minute incubation at 25°C)

storage temp.

−20°C

General description

The PHOS-Select™ iron affinity gel is a novel iron [Fe(III)] chelate matrix. This matrix provides high capacity affinity binding of molecules containing phosphate groups.

Application

Phos-Select™ products facilitate a number of applications including:
  • affinity enrichment of phosphopeptides obtained from protein tryptic digests
  • direct transfer of phosphocompounds for analysis (HPLC or mass spectrometry)
  • enrichment of phosphopeptides by immobilized metal affinity chromatography (IMAC)

Physical form

aqueous glycerol suspension

Other Notes

Phos-Select™ Iron Affinity Gel is a novel iron [Fe(III)] chelate matrix based on Sigma′s NTA analog chelate ligand. The matrix provides high capacity affinity binding of molecules containing phosphate groups.

Legal Information

PHOS-Select is a trademark of Sigma-Aldrich Co. LLC

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This Item
H8162H0537I1408
PHOS-Select™ Iron Affinity Gel

Sigma-Aldrich

P9740

PHOS-Select™ Iron Affinity Gel

HIS-Select® Cobalt Affinity Gel (1:1 suspension in a 30% ethanol solution)

Millipore

H8162

HIS-Select® Cobalt Affinity Gel

HIS-Select® HF Nickel Affinity Gel

Millipore

H0537

HIS-Select® HF Nickel Affinity Gel

IMAC-Select Affinity Gel (1:1 suspension in a 30% ethanol solution)

Sigma-Aldrich

I1408

IMAC-Select Affinity Gel

form

suspension

form

(1:1 suspension in a 30% ethanol solution)

form

suspension

form

(1:1 suspension in a 30% ethanol solution)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

pH

~5

pH

-

pH

-

pH

-

capacity

≥2 μmol/mL, gel (phosphopeptide)(following a 30 minute incubation at 25°C)

capacity

>15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)

capacity

15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

capacity

10-30 μmol/mL binding capacity (Cu+2)

shelf life

2 yr at −20‑0 °C

shelf life

1 yr (when stored properly.)

shelf life

-

shelf life

1 yr

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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