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P2192

10X PCR Buffer

Optimized for routine PCR with MgCl2 included

Synonym(s):

PCR Buffer Solution, PCR Reaction Buffer

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About This Item

NACRES:
NA.52
UNSPSC Code:
41106306
Pricing and availability is not currently available.
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Quality Segment

300

form

liquid

packaging

vial of 1.5 mL

technique(s)

PCR: suitable, whole genome amplification: suitable

color

colorless

application(s)

agriculture

foreign activity

DNase, RNase, none detected

storage temp.

−20°C

Application

10X PCR Buffer has been used as a component of the PCR mixture:
  • for the amplification of viral DNA
  • to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals
  • to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.

Features and Benefits

  • Standalone buffer
  • Compatible with JumpStart™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)

Preparation Note

Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
P2317L6669S5193
10X PCR Buffer Optimized for routine PCR with MgCl2 included

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P2192

10X PCR Buffer

10X PCR Buffer without MgCl2 Optimized for routine PCR without MgCl2

Sigma-Aldrich

P2317

10X PCR Buffer without MgCl2

LuminoCt® qPCR ReadyMix™ For fast probe-based quantitative PCR

Sigma-Aldrich

L6669

LuminoCt® qPCR ReadyMix™

SYBR™ Green JumpStart™ Taq ReadyMix™ for quantitative PCR, without MgCl2 in buffer

Sigma-Aldrich

S5193

SYBR™ Green JumpStart™ Taq ReadyMix™

description

Optimized for routine PCR with MgCl2 included

description

Optimized for routine PCR without MgCl2

description

For fast probe-based quantitative PCR

description

for quantitative PCR, without MgCl2 in buffer

technique(s)

PCR: suitable, whole genome amplification: suitable

technique(s)

whole genome amplification: suitable, PCR: suitable

technique(s)

qPCR: suitable, whole genome amplification: suitable, RT-qPCR: suitable

technique(s)

whole genome amplification: suitable, RT-qPCR: suitable

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

color

colorless

color

colorless

color

colorless

color

colorless

foreign activity

DNase, RNase, none detected

foreign activity

-

foreign activity

-

foreign activity

-

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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