E0887

Esterase from rabbit liver

lyophilized powder, ≥30 units/mg protein

• Synonym(s): Carboxyl esterase, Carboxylic-ester hydrolase
• CAS Number: 9016-18-6
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-773-7
• MDL number: MFCD00131030
• EC Number: 3.1.1.1 (BRENDA, IUBMB)
• Specific activity: ≥30 units/mg protein

Properties

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥30 units/mg protein
• mol wt: 60 kDa
• composition: Protein, ≥85% biuret
• storage temp.: −20°C

Description

General description

Esterase belongs to the C-family of carboxylesterases. It is a glycoprotein, mainly present in the endoplasmic reticulum of hepatocytes.

Application

The enzyme from Sigma has been used to study the effect of divalent metal ions on the activity of esterase.

Esterase from rabbit liver has been used:

• in comparative biophysical studies with bovine bile-salt-activated lipase in the assays with vinyl acetate, triacylglycerols, p-nitrophenyl esters and serine enzyme inhibitors
• in the catalysis and kinetic profiling studies of peptidic compounds
• in in vitro stability assays of prodrug compounds (BMS-292655 and BMS300043)
• in the hydrolysis of oleuropein aglycone (Ole-AG)

Esterase from rabbit liver has been used in a study to investigate a toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used in a study to investigate the effect of simvastatin on expression and activity of a lipoprotein-associated phospholipase A(2).

Biochem/physiol Actions

Esterase enzyme catalyzes hydrolysis of carboxylic ester to carboxylate and alcohol in the presence of water. Rabbit liver carboxylesterase catalyzes enantioselective hydrolysis of cineol derivatives.

Esterase enzyme has broad substrate specificity. Esterase from rabbit liver has been used to investigate the toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used for the hydrolysis of simvastatin.

Physical form

Lyophilized powder containing Tris buffer salts

Other Notes

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)