KG1a
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91030101, human bone marrow, Lymphoblast
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About This Item
UNSPSC Code:
41106514
Recommended Products
biological source
human bone marrow
packaging
tube of 5 μg 91030101-DNA-5UG
pkg of vial of cells 91030101-1VL
growth mode
Suspension
karyotype
2n = 46, pseudodiploid
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian bone marrow acute myelogenous leukaemia
Cell Line Description
Derived from KG-1, however KG1a is unresponsive to colony stimulating factor in soft agar culture and does not express the Ia-like antigen. KG1a is resistant to phorbol-diester induced macrophage differentiation and cell proliferation. KG1a cells are morphologically, cytochemically and functionally less mature than the parental KG-1. Expresses HLA A30,31; B35; CW4.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Culture Medium
IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 5x100,000 to 1,000,000 cells/ml. Do not culture below 100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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