This product contains EDTA as the major active component, which acts as a chelating agent for divalent cations to loosen cell–cell contacts but does not break down the extracellular matrix as trypsin does. Empirical testing is needed to determine if this approach is more effective. It is highly recommended to review the literature to determine if an EDTA-only dissociation method has been successfully used with the particular primary microglia preparation protocol.
C5789
Cell Dissociation Solution Non-enzymatic 1x
Prepared in Hanks′ Balanced Salt Solution without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
Cell Dissociation Reagent
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Quality Level
sterility
sterile-filtered
product line
BioReagent
form
solution
concentration
1 ×
technique(s)
cell culture | mammalian: suitable
pH
6.5-7.5
shipped in
wet ice
storage temp.
room temp
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General description
Cell Dissociation Solution Non-enzymatic buffer systems are alternative to the canonical trypsin based buffers for dissociation of adherent mesenchymal stem cell (MSC) monolayers.[1]
Application
Cell Dissociation Solution Non-enzymatic 1x has been used in the dissociation of murine macrophage (RAW2647) cells,[2] A549 pulmonary cells,[3] islets cells[4] and 2D cell and 3D mammospheres.[5]
Special non-enzymatic formulation for gently dislodging adherent cell types from plastic or glass surfaces.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
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Hi, I am working with primary microglia and it very tricky to dissociate. I usually dissociate them with 0.5% Trypsin for 7 min at 37˚C and still hard to collect them. May I know whether this reagent would be a better alternative? Thank you!
1 answer-
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What is the concentration of EDTA in Cat#C5789? Thanks
1 answer-
The concentration of EDTA in this formulation is proprietary. Additional components include Sodium Citrate, Nitrilotriacetic Acid, and Glycerol in Hank's Balanced Salt solution. Should the concentration of EDTA be required, product E8008, EDTA 0.2% in DPBS, may be of interest. Both items are tested for cell dissociation. Please see the link below to review this product option:
https://www.sigmaaldrich.com/US/en/product/sigma/e8008Helpful?
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