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B7024

Sigma-Aldrich

Anti-Goat IgG (whole molecule)–Biotin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
MFCD00162346
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

goat

technique(s)

direct ELISA: 1:20,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

The antibody binds to all goat Igs.

Application

Anti-Goat IgG (whole molecule)-Biotin antibody produced in rabbit has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemistry
  • immunofluorescence analysis

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to Anti-Goat IgG (whole molecule) antibody allows for the binding of various labels such as avidin or streptavidin.

Other Notes

Antibody adsorbed with human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Adsorbed to reduce background staining with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Immunoglobulin G, complement factor C3 and lymphocytes in proliferative intraocular disorders
Weller M, et al.
International Ophthalmology, 14(4), 277-283 (1990)
J Martin Wild
The Journal of comparative neurology, 525(7), 1731-1742 (2017-01-13)
The dorsal pallidum in birds is considered similar, if not homologous, to the globus pallidus (GP) of mammals. The dorsal pallidum projects to both thalamic and midbrain targets similar to the direct and indirect pathways arising from the internal and
Regional and cellular distribution of ephrin-B1 in adult mouse brain
Migani P, et al.
Brain Research, 1247(4), 50-61 (2009)
A real-time immuno-PCR assay for routine ultrasensitive quantification of proteins
Adler M, et al.
Biochemical and Biophysical Research Communications, 308(2), 240-250 (2003)
Elina V García et al.
Theriogenology, 81(8), 1032-1041 (2014-03-04)
Bone morphogenetic proteins (BMPs) play a crucial role in mammalian reproduction, but little is known about their expression and function in the oviduct, where preimplantation events take place. In the present study, messenger RNA (mRNA) expression of BMPs was examined
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