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88190

Sigma-Aldrich

Tetrazolium Blue Chloride

≥90% (T), powder, suitable for microbiology

Synonym(s):

3,3′-(3,3′-Dimethoxy[1,1′-biphenyl]-4,4′-diyl)-bis(2,5-diphenyl-2H-tetrazolium) dichloride, 3,3′-Dianisole-4,4′-bis(3,5-diphenyltetrazolium chloride), BTC, Blue Tetrazolium chloride, TZ

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About This Item

Empirical Formula (Hill Notation):
C40H32Cl2N8O2
CAS Number:
Molecular Weight:
727.64
Beilstein:
3894077
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83

product name

Tetrazolium Blue Chloride, indicator for germination, suitable for microbiology, ≥90% (T)

grade

indicator for germination

Quality Level

Assay

≥90% (T)

form

powder

mp

255 °C (dec.) (lit.)

solubility

methanol: 0.5 g/10 mL ( hot)

application(s)

microbiology

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccccc3)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccccc7)-c8ccccc8

InChI

1S/C40H32N8O2.2ClH/c1-49-37-27-31(23-25-35(37)47-43-39(29-15-7-3-8-16-29)41-45(47)33-19-11-5-12-20-33)32-24-26-36(38(28-32)50-2)48-44-40(30-17-9-4-10-18-30)42-46(48)34-21-13-6-14-22-34;;/h3-28H,1-2H3;2*1H/q+2;;/p-2

InChI key

MUUHXGOJWVMBDY-UHFFFAOYSA-L

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Other Notes

Used in the assay of reducing sugars; Used in the preparation of DNA-cation complexes, immunoadsorbents for anti-DNA antibodies.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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J.P. Robin et al.
Sciences Des Aliments, 1, 233-233 (1981)
Tetrazolium-based colorimetric assay for the detection of HIV replication inhibitors: revisited 20 years later.
Pannecouque C, Daelemans D, and De Clercq E
Nature Protocols, 3(3), 427-434 (2008)
V A DeBari et al.
Journal of immunological methods, 40(1), 89-94 (1981-01-01)
DNA can be precipitated as complexes with metal and organic cations. We have prepared these products using divalent metal cations (Cu2+, Mn2+, Ni2+, Zn2+, and Co2+) and ditetrazolium ions (blue tetrazolium and neotetrazolium). They are effective adsorbents, in vitro, for
Chin-I Chang et al.
Sensors (Basel, Switzerland), 12(3), 2710-2728 (2012-06-28)
We coupled 16S rDNA PCR and DNA hybridization technology to construct a microarray for simultaneous detection and discrimination of eight fish pathogens (Aeromonas hydrophila, Edwardsiella tarda, Flavobacterium columnare, Lactococcus garvieae, Photobacterium damselae, Pseudomonas anguilliseptica, Streptococcus iniae and Vibrio anguillarum) commonly
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Nature protocols, 3(3), 427-434 (2008-03-08)
Since its first description 20 years ago, the tetrazolium-based colorimetric (MTT) assay using MT-4 cells for the detection of anti-HIV compounds has been widely used. This method, which remains popular, provides more information than more recently developed methods and, therefore

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