Skip to Content
Merck
All Photos(1)

Documents

77730

Millipore

Gram Staining Kit

for microscopy, used for differentiation of bacteria on the basis of their gram nature.

Synonym(s):

Bacteria Staining Kit according to Gram

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41171621
NACRES:
NA.85

grade

for microscopy

Quality Level

100

Agency

according to GB 4789.30-2016

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

clinical testing
environmental
food and beverages
pharmaceutical

suitability

bacteria

General description

The Gram staining kit is used in differential staining technique that is most widely applied in all microbiology disciplines laboratories. It is one of the most important criteria in any identification scheme for all types of bacterial isolates. There are many physiological differences between gram-positive and gram-negative cell walls. Ever since Christian Gram has discovered Gram staining, this process has been extensively investigated and redefined. In practice, a thin smear of bacterial cells is stained with crystal violet, then treated with iodine containing mordant to increase the binding of the primary stain. A decolorizing solution of alcohol or acetone is used to remove the crystal violet from cells which bind it weakly and then the counterstain safranin is used to provide color contrast in those cells that are decolorized. In a properly stained smear by gram staining procedure, the gram-positive bacteria appear blue to purple and gram-negative cells appear pink to red

Application

The Gram staining method is one of the most important staining techniques in microbiology. It is also the most important criteria in any identification scheme for all types of bacterial isolates to differentiate the gram-positive and gram-negative bacteria.

Components

Kit contains (250ml each):
94448 Gram′s crystal violet Solution
90107 Gram′s iodine Solution
75482 Gram′s Decolorizer Solution
94635 Gram′s safranin Solution

Signal Word

Danger

Hazard Statements

H225,H319,H336,H351,H412

Hazard Classifications

Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

Supplementary Hazards

EUH066

Storage Class Code

3 - Flammable liquids

Flash Point(F)

68.0 °F

Flash Point(C)

20 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCM2797
BCCM0267
BCCL8176
BCCL6405
BCCL5052

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Hiral Mistry et al.
PloS one, 11(9), e0162256-e0162256 (2016-09-08)
Bovine mastitis caused by multidrug resistant Staphylococcus aureus is a huge problem reported worldwide, resulting in prolonged antibiotic treatment and death of livestock. The current study is focused on surveillance of antibiotic susceptibility along with genotypic and phenotypic characterization of
Potential Threapeutic Applications of Extract Made from Electroporated Xenopus
Laevis frog Oocytes in Murine Models of Melanoma, Traumatic Brain Injury and
Experimental Skin Wrinkling
Sergei Paylian
Bjog : An International Journal of Obstetrics And Gynaecology (2016)
U E Rodríguez-Castrejón et al.
Archives of microbiology, 204(3), 191-191 (2022-02-24)
The study of arsenic (As)-resistant microorganisms with high As removal capacity is fundamental for the development of economically sustainable technologies used for the treatment of water contaminated with metalloid. In the current study, four bacterial strains were isolated from As-contaminated
Prevalence of blaOXA-?-Lactamase-Producing and Methicillin-Resistant Staphylococcus aureus in Iran
Navidinia M
AANA Journal (2016)
Wasim Sajjad et al.
Journal of basic microbiology, 59(3), 323-336 (2018-12-29)
The exploration of microbial diversity in extremely acidic habitats has provided a vital base for the progression of minerals biotechnology. Three indigenous iron-oxidizing acidophilic bacterial strains were isolated through serial dilution of enriched bacterial culture from Baiyin Copper Mine Stope
View All Related Papers

Articles

Vibrio Detection

Vibrios are motile, curved or comma-shaped bacilli and have a single polar flagella with sheet proteins. They are often found in open water, freshwater and saltwater.

Clostridia Diagnostic

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism.

Clostridia Diagnostic

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism.

Clostridia Diagnostic

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism.

See All

Protocols

Listeria: Surviving Bacteria

Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service