SCC050

PluriStem C57BL/6N Murine ES Cells

This Murine Embryonic Stem Cell line is derived from C57BL/6N blastocysts.

• UNSPSC Code: 41106509
• NACRES: NA.81
• eCl@ss: 32011203
• Biological source: mouse

Properties

• biological source: mouse
• Quality Segment: 100
• manufacturer/tradename: PluriSTEM^®
• technique(s): cell culture | stem cell: suitable
• input: sample type: mouse embryonic stem cell(s)
• shipped in: liquid nitrogen
• storage temp.: -140 to -196°C

Description

General description

Embryonic Stem Cell line derived from C57BL/6N blastocysts. C57BL/6N is the most commonly used inbred mouse strain. Large scale mouse knockout projects such as NIH’s KOMP project use C57BL/6 as the standard background for mouse genome engineering. C57BL/6 is also the reference strain for mouse genome sequencing at the NHGRI. C57BL/6N mice show good breeding performance. As a commonly used reference strain, transgenic mouse models are often backcrossed to a uniform C57BL/6 background, and B6 ES cells facilitate building complex models which combine gene inactivation with existing transgenics maintained in a B6 background. Additional phenotypic information is available at the Mouse Genome Informatics website (http://www.informatics.jax.org/external/festing/mouse/docs/C57BL.shtml). The B6 ES line was genome verified by determining the alleles at 29 SNPs that are informative across a large number of inbred mouse strains.

STRAIN: C57BL/6N

GENDER: Male

KARYOTYPE: 40XY

Application

Research Category
Stem Cell Research

This Murine Embryonic Stem Cell line is derived from C57BL/6N blastocysts.

Biochem/physiol Actions

Embryonic Stem Cells

Packaging

2.5 x 10⁶ cells per vial, passage 9

Physical form

Cells are supplied frozen in basal medium supplemented with 50% FCS and 10% DMSO

Preparation Note

Place vials in liquid nitrogen immediately upon receipt until it is convenient to proceed to subculture.

Other Notes

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

Legal Information

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable