MABE1987
Anti-LINE1 ORF2 Antibody, clone 1G4E11
Synonym(s):
EC:2.7.7.49;ORF2p endonuclease;ORF2p
About This Item
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
1G4E11, monoclonal
mol wt
calculated mol wt 149.01 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 100 μL
technique(s)
ELISA: suitable
inhibition assay: suitable
western blot: suitable
isotype
IgG1
epitope sequence
N-terminal
UniProt accession no.
storage temp.
-10 to -25°C
target post-translational modification
unmodified
Gene Information
human ... Orf2p(0)
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in lysate from HEK293 cells overexpressing human ORF2p EN.
Western Blotting Analysis: A 1:500 dilution of this antibody detected LINE1 ORF2 in lysate from HEK293 cells overexpressing human ORF2p EN.
Tested applications
Western Blotting Analysis: A representative lot detected LINE1 ORF2 in Western Blotting applications (Sokolowski, M. et al. (2014). Mob DNA. 5(1):29.).
Inhibition Analysis: A representative lot partially inhibited L1 endonuclease activity in an in vitro endonuclease cleavage assay. (Sokolowski, M. et al. (2014). Mob DNA. 5(1):29.).
ELISA Analysis: A representative lot detected LINE1 ORF2 in ELISA applications (Sokolowski, M. et al. (2014). Mob DNA. 5(1):29.).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Physical form
Reconstitution
Storage and Stability
Other Notes
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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