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Key Documents

MABC1813

Sigma-Aldrich

Anti-CD44v5 Antibody, clone C44Mab-3

Synonym(s):

CDw44, Epican, Extracellular matrix receptor III (ECMR-III) GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, Phagocytic glycoprotein 1 (PGP-1), Phagocytic glycoprotein I (PGP-I)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

C44Mab-3, monoclonal

mol wt

calculated mol wt 82 kDa
observed mol wt ~N/A kDa

purified by

using protein G

species reactivity

human

species reactivity (predicted by homology)

monkey

packaging

antibody small pack of 100

technique(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

epitope sequence

N-terminal half

Protein ID accession no.

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... CD44(960)

Specificity

Clone C44Mab-3 is a mouse monoclonal antibody that detects CD44v5. It targets an epitope within 30 amino acids from the N-terminal half of the protein.

Immunogen

CHO cells transfected with PA16-tagged CD44v3 10.

Application

Quality Control Testing

Evaluated by Flow Cytometry in a CHO cells overexpressing CD44v3-10.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD44v6 in a one million CHO cells overexpressing CD44v3-10.

Tested Applications

Western Blotting Analysis: A representative lot detected CD44v5 in Western Blotting applications (Kudo, Y., et al. (2023). Antibodies (Basel). 12(2):31).

Immunohistochemistry Applications: A representative lot detected CD44v5 in Immunohistochemistry applications (Kudo, Y., et al. (2023). Antibodies (Basel). 12(2):31).

Flow Cytometry Analysis: A representative lot detected CD44v5 in Flow Cytometry applications (Kudo, Y., et al. (2023). Antibodies (Basel). 12(2):31).

ELISA Analysis: A representative lot detected CD44v5 in ELISA applications (Kudo, Y., et al. (2023). Antibodies (Basel). 12(2):31).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

CD44 antigen (UniProt: P16070; also known as CDw44, Epican, Extracellular matrix receptor III (ECMR-III), GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1 (PGP-1), Phagocytic glycoprotein I (PGP-I)) is encoded by the CD44 (also known as LHR, MDU2, MDU3, MIC4) gene (Gene ID: 960) in human. CD44 is a Type 1 transmembrane glycoprotein involved in cell adhesion, migration, and proliferation. It also plays a role in lymphocyte activation, tumor metastasis, and wound healing. It is encoded by the CD44 gene, and it is expressed in a wide range of cell types. The protein′s structure includes a hyaluronic acid (HA) binding domain, a stem region, and a variable extracellular domain. Alternative splicing may produce 19 isoforms. CD44 splice variants (CD44v) play a role in tumorigenesis and malignancy. It is also implicated in various other diseases, including autoimmune disorders, cardiovascular diseases and fibrosis.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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