FCMAB101A4
Milli-Mark® Anti-BrdU Antibody, clone BU-1 Alexa Fluor™ 488 conjugated
clone Bu-1, Milli-Mark®, from mouse
Synonym(s):
BrdU
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Bu-1, monoclonal
species reactivity
human
species reactivity (predicted by homology)
all
manufacturer/tradename
Milli-Mark®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
isotype
IgG2a
shipped in
wet ice
target post-translational modification
unmodified
General description
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. This antibody does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU. The antibody′s special ability is thought to be due to the presence of DNAse in the antibody solution arising from the mycoplasma infecting the original hybridoma line {Gonchoroff, 1985}.
Specificity
Specifically binds to bromodeoxyuridine (crossreacts with iodouridine 10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.
Immunogen
Bromodeoxyuridine
Application
Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor 488 conjugated detects level of BrdU & has been published & validated for use in FC & IC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Quality
Evaluated by flow cytometry with Jurkat Cells.
Target description
none given
Physical form
Presentation needs to say: Purified mouse monoclonal IgG2a supernatant conjugated to Alexa Fluor™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/transmission.
Protein G Purified
Storage and Stability
Stable for 6 months at 2-8°C from date of receipt. Protect from light.
Analysis Note
Control
After incorporation of BrdU, all DNA containing species
After incorporation of BrdU, all DNA containing species
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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