1.16177

LiChrospher^® 100 RP-18 (5 µm)

HPLC sorbent

• Synonym(s): TM=["LiChrospher"] C18 sorbent for HPLC
• UNSPSC Code: 41115709
• NACRES: SB.52
• Particle size: 5 μm
• Matrix active group: C18 (RP18), C18 bonding phase
• Pore size: 100 Å pore size
• Matrix: fully porous particle

Properties

• Product Name: LiChrospher^® 100 RP-18 (5 µm),
• material: stainless steel column
• agency: suitable for USP L1
• product line: LiChrospher^®
• feature: endcapped: no
• analyte functional class(es): antibiotics, explosives, fat soluble vitamins, lipids, nutraceuticals and antioxidants, oils, pigments, sweeteners, water soluble vitamins
• analyte chemical class(es): PAHs, PCBs (aroclor identification), PFAS, aflatoxins, alcohols / fermentation, alcohols and aldehyde, aliphatic amines, alkaloids, amino acids, aromatic amines, cannabinoids as tms derivatives, carotenoids, catecholamines, coloring compounds, essential oils, esters, fatty acids, flavonoids, glycans, ketones, metabolized steroids, nitrosamines, nucleic acids, nucleosides, oligonucleotides, organic phosphates, peptides, pesticides, phenols, phospholipids, phthalate esters, preservatives, proteins, steroids (cholesteryl esters), sulfonamides
• packaging: 1 ea of
• extent of labeling: 21% Carbon loading
• parameter: 60 °C max. temp.
• technique(s): HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium)
• surface area: 350 m²/g
• matrix: fully porous particle
• matrix active group: C18 (RP18), C18 bonding phase
• particle size: 5 μm
• pore size: 100 Å pore size
• operating pH range: 2-7.5
• bulk density: 200‑400 kg/m³
• separation technique: reversed phase
• storage temp.: no temp limit

Description

General description

LiChrospher^® RP-18 is a reliable and versatile traditionally produced spherical silica gel carrier with C-18 reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds.

Application

• Fast Determination of a Novel Iron Chelate Prototype Used as a Fertilizer by Liquid Chromatography Coupled to a Diode Array Detector.: This study explores the use of LiChrospher^® 100 RP-18 (5 µm) for the rapid analysis of a new iron chelate fertilizer, showcasing its effectiveness in agricultural applications (Valverde et al., 2021).


• Application of Chemometric Approach for Development and Validation of High-Performance Liquid Chromatography Method for Estimation of Ropinirole Hydrochloride.: The research utilizes LiChrospher^® 100 RP-18 (5 µm) for the quantification of ropinirole in pharmaceutical formulations, highlighting its precision and robustness (Fatima et al., 2019).


• Quantification of Alprenolol and Propranolol in Human Plasma Using a Two-Dimensional Liquid Chromatography (2D-LC).: This paper demonstrates the application of LiChrospher^® 100 RP-18 (5 µm) in clinical settings for the analysis of beta-blockers in plasma, indicating its utility in pharmacokinetics (Gonçalves et al., 2017).


• Ultrasound-Assisted Surfactant-Enhanced Emulsification Micro-Extraction Followed by HPLC for Determination of Preservatives in Water, Beverages, and Personal Care Products.: The study highlights the use of LiChrospher^® 100 RP-18 (5 µm) for environmental and quality control analyses, demonstrating its versatility in various matrices (Jan-E et al., 2017).


• Development and Validation of Stability-Indicating HPLC Method for Determination of Cefpirome Sulfate.: This research focuses on the stability testing of cefpirome using LiChrospher^® 100 RP-18 (5 µm), emphasizing its reliability for pharmaceutical quality control (Zalewski et al., 2014).

Analysis Note

Application test: passes test
Pressure: ≤ 35 bar
Theoretical Plates (N/m) (Toluene): ≥ 60000
stability testing (performance): passes test
separation factor (Acetophenone/Phenol, selectivity test): 2.05 - 2.25
separation factor (Acetophenone/Cinnamic acid, selectivity test): 1.24 - 1.37
capacity factor (Toluene): 13.0 - 18.0

Legal Information

LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable