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Column	Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 5 µm particles (50773-U)
Column Temp.	30 °C
Mobile Phase	[A] 0.02 M Ammonium Formate, pH = 3.0; [B] Acetonitrile
Gradient	0-50% B in 1 min. Hold at 50% B until 1.5 min
Flow Rate	1.5 mL/min
Pressure	1639 psi (113 bar)
Sample	Dissolved in 50/50 water/ACN
Injection	1 µL
Detector	UV, 276 nm

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Categories	Analytical Chromatography, Vitamins and Natural products
Featured Industry	Vitamins, Nutraceuticals, and Natural Products
Legal Information	Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Suitability	Application for HPLC

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HPLC Analysis of Vitamins B1, B6 and B12 Using Ascentis® Express RP-Amide

Conditions

Description

HPLC Analysis of Vitamins B1, B6 and B12 Using Ascentis^® Express RP-Amide