- Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice.
Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice.
Scientific reports (2017-10-19)
Mohamed Abdo Rizk, Shimaa Abd El-Salam El-Sayed, Mahmoud AbouLaila, Rasha Eltaysh, Naoaki Yokoyama, Ikuo Igarashi
PMID29038534
ABSTRACT
In this study, we evaluated the validity of a fluorescence-based assay using SYBR Green I (SG I) stain for screening antibabesial compounds against B. microti in mice. Two different hematocrits (HCTs; 2.5% and 5%) were used. Correlating relative fluorescence units (RFUs) with parasitemia showed significant linear relationships with R
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