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Mutational biosynthesis of a FK506 analogue containing a non-natural starter unit.

Molecular bioSystems (2012-12-12)
Yeon Hee Ban, Jong Hyun Lee, Gyo Rim Gu, Boram Lee, SangJoon Mo, Ho Jeong Kwon, Yeo Joon Yoon
ABSTRACT

A FK506 analogue containing a non-natural starter unit was obtained through mutasynthesis by feeding cultures of Streptomyces sp. KCTC 11604BP fkbO deletion mutant with 3-cyclohexene-1-carboxylic acid. The structure of the new compound, 32-dehydroxy-FK506, and its biological activities were determined.

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Sigma-Aldrich
3-Cyclohexene-1-carboxylic acid, 97%