Metabolic activation of CaMKII by coenzyme A.

Metabolic activation of CaMKII by coenzyme A.

Molecular cell (2013-10-08)

Francis McCoy, Rashid Darbandi, Hoi Chang Lee, Kavitha Bharatham, Tudor Moldoveanu, Christy R Grace, Keela Dodd, Wenwei Lin, Si-Ing Chen, Rajendra P Tangallapally, Manabu Kurokawa, Richard E Lee, Anang A Shelat, Taosheng Chen, Douglas R Green, Robert A Harris, Sue-Hwa Lin, Rafael A Fissore, Roger J Colbran, Leta K Nutt

PMID24095281

ABSTRACT

Active metabolism regulates oocyte cell death via calcium/calmodulin-dependent protein kinase II (CaMKII)-mediated phosphorylation of caspase-2, but the link between metabolic activity and CaMKII is poorly understood. Here we identify coenzyme A (CoA) as the key metabolic signal that inhibits Xenopus laevis oocyte apoptosis by directly activating CaMKII. We found that CoA directly binds to the CaMKII regulatory domain in the absence of Ca(2+) to activate CaMKII in a calmodulin-dependent manner. Furthermore, we show that CoA inhibits apoptosis not only in X. laevis oocytes but also in Murine oocytes. These findings uncover a direct mechanism of CaMKII regulation by metabolism and further highlight the importance of metabolism in preserving oocyte viability.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

C4282

Sigma-Aldrich

Coenzyme A hydrate, ≥85% (UV, HPLC)