Simultaneous determination of residues of florfenicol and the metabolite florfenicol amine in fish tissues by high-performance liquid chromatography.

A simple and rapid high-performance liquid chromatographic method for the simultaneous extraction and determination of residues of florfenicol and its metabolite florfenicol amine in fish tissue, muscle and liver has been developed. The calibration curves were linear, the recovery of florfenicol was 99-107%, and the recovery of florfenicol amine was 94-100%. The detection limits for florfenicol and florfenicol amine were 20 ng/g in muscle and 50 ng/g in liver.