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Pirahy virus: Identification of a new and potential emerging arbovirus in South Brazil.

Virus evolution (2022-03-22)
Marcel Kruchelski Tschá, Andreia A Suzukawa, Gabriela Flavia Rodrigues-Luiz, Allan Martins da Silva, Allan Henrique Depieri Cataneo, Gabriela Mattoso Coelho, Adão Celestino Ferreira, Lia Carolina Soares Medeiros, Daniel Mansur, Camila Zanluca, Claudia N Duarte Dos Santos
ABSTRACT

Genomic and epidemiological surveillance are paramount for the discovery of new viruses with the potential to cross species barriers. Here, we present a new member of the genus Alphavirus found in Trichoprosopon and Wyeomia mosquitoes, tentatively named Pirahy virus (PIRAV). PIRAV was isolated from mosquito pools collected in a rural area of Piraí do Sul, South Brazil. In vitro assays revealed that PIRAV replicates and causes cytopathic effects in vertebrate cell lines such as Vero E6, SH-SY5Y, BHK-21 and UMNSAH/DF-1. Genomic signature analysis supports these results showing a dinucleotide and codon usage balance compatible with several hosts. Phylogenetic analyses placed PIRAV basal to the Venezuelan equine encephalitis complex. Genome analyses, electron microscopy, and biological characterization show findings that may alert for the emergence of a new arbovirus in South America.

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Sigma-Aldrich
Anti-Eastern Equine Encephalitis Antibody, Virus, clone 1A4B.6, clone 1A4B.6, Chemicon®, from mouse