TBST for Western Blotting
Tris-buffered saline with 0.1% Tween® 20 detergent (TBST) is an effective wash buffer for many immunoassays. To make 1 L of TBST wash buffer, add 100 mL of 10X TBS and 1 mL Tween® 20 detergent to 900 mL of water.
This calculator enables the accurate preparation of a 1X TBST working solution whether you are making enough for a single experiment or for the entire lab. Input your desired volume, click the CALCULATE button, and the table will populate with the amount of each component needed.
1X Solution concentrations:
- Tris: 20 mM
- NaCl: 150 mM
- Tween® 20 detergent: 0.1% (w/v)
TBST Recipe Calculator
OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS & SOLUTIONS
Stock Solutions
- 4X SDS Sample Loading Buffer
- 10X Phosphate-Buffered Saline (PBS)
- 10X Tris-Buffered Saline (TBS) Stock Solution
- Stock Components for Western Blot Stripping Solutions
Wash & Blocking Buffers
- 1X Tris-Buffered Saline (TBS) Working Solution
- 1X Phosphate-Buffered Saline (PBS) Recipe Calculator
- 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
- Non-Fat Dry Milk (NFDM) Blocking Buffer
- Bovine Serum Albumin (BSA) Blocking Buffer
Stripping Solutions
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