Y2001

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, tryptophan and uracil

• Sinonimo/i: Synthetic drop-out medium for yeast
• Numero MDL: MFCD01095679
• Codice UNSPSC: 12000000
• NACRES: ND.02

Proprietà

• Livello qualitativo: 100
• Forma fisica: powder
• applicazioni: food and beverages

Descrizione

Applicazioni

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Componenti

The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for histidine, leucine, tryptophan and uracil. Each amino acid is present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L)

Altre note

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Quantità

1.4 g will supplement 1 liter of medium.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)