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TRUE7

Sigma-Aldrich

TrueGel3D Hydrogel Kits

SLO-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.71

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

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Categorie correlate

Descrizione generale

The gel is formed by stable thioether bonds between thiol groups on CD cell-degradable crosslinker and thio-reactive groups on SLO-DEXTRAN. The polymer, SLO-DEXTRAN is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-DEXTRAN. SLO-Dextran does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1UM) prior to the crosslinking step.

The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

Applicazioni

TRUE7-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of mechanical stimuli on cell physiology and behavior. The SLO-DEXTRAN based hydrogels are used in microchannels and also in syringes for injection. These hydrogels are also employed to study cell migration of neutrophils, dendritic cells and lymphocytes.

Caratteristiche e vantaggi

  • Slow gelling (takes 2.5 minutes to 6 minutes, depends on gel stiffness)
  • Flexibility in modulating gel stiffness
  • Contains MMP-cleavable crosslinker
  • Allow cell recovery and post-culture analyses
  • Cell inert hydrogel (if not customized with RGD peptide)

Componenti

TRUE7-1KT contains SLO-DEXTRAN solution in phosphate buffer and lyophilized CD cell-degradable crosslinker with reconstitution buffer and water.

Avvertenza

Avoid prolonged exposure of CD cell-degradable crosslinker to air.

Pittogrammi

Corrosion
GHS05

Avvertenze

Warning

Indicazioni di pericolo

H290,H315,H319

Classi di pericolo

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Certificati d'analisi (COA)

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Omid Mashinchian et al.
eLife, 11 (2022-03-05)
Sustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specific cell populations. Here, we describe a

Articoli

True Gel3D™ Hydrogel Troubleshooting

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Hydrogels for 3D Cell Culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

Protocolli

TrueGel3D™ Hydrogel Prep Protocol

TrueGel3D™ is a chemically defined hydrogel for 3D cell growth, facilitating microchannel filling with slow gelation.

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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