P1869
Anti-Phosphotyrosine antibody, Mouse monoclonal
clone pT-154, purified from hybridoma cell culture
Sinonimo/i:
Phospho-Tyr, Phospho-tyrosine, p-Tyr
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About This Item
Prodotti consigliati
Coniugato
unconjugated
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
pT-154, monoclonal
Concentrazione
~2 mg/mL
tecniche
direct ELISA: suitable
immunohistochemistry: suitable
microarray: suitable
western blot: 2-4 μg/mL using total extract of A431 cells stimulated by EGF
Isotipo
IgG2b
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Categorie correlate
Descrizione generale
Anti-Phosphotyrosine antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the pT-154 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a tyrosine phosphorylated peptide. Members of protein-tyrosine kinases (PTKs) family consists of extra-cellular domain that is responsible for ligand binding, a trans-membrane domain, modular domain and an intracellular domain.
Immunogeno
tyrosine phosphorylated peptide.
Applicazioni
Anti-Phosphotyrosine antibody, Mouse monoclonal has been used in:
- enzyme linked immunosorbent assay (ELISA)
- western blot
- immunohistochemistry
Azioni biochim/fisiol
Protein phosphorylation is a post-translational modification that regulates signal transduction. Phosphorylation of tyrosine residues in proteins has been associated with oncogenesis, cell growth and survival . Monoclonal Anti-Phosphotyrosine antibody is specific for rat and human proteins/peptides that contain phosphotyrosine residues. As determined by competitive ELISA, the product does not bind to non-phosphorylated tyrosine, phosphorylated serine or threonine, ATP or AMP.
Protein-tyrosine kinases (PTKs) are enzymes that catalyze the transfer of Γ-phosphate of ATP to tyrosine residues of protein substrates. The PTKs are responsible for many biological processes like cell cycle, proliferation, oncogenesis and development. They are tightly regulated by other kinases and by autophosphorylation activity. Monoclonal antibodies specific for phosphotyrosine are essential tools for the study of tyrosine phosphorylation in post-translational modification in many biological processes.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Protein tyrosine kinase structure and function
Hubbard SR and Till JH
Annual Review of Biochemistry, 69(1), 373-398 (2000)
Shashikant Gupta et al.
Andrologia, 54(1), e14255-e14255 (2021-09-29)
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Tyrosine kinase-role and significance in cancer
Paul MK and Mukhopadhyay AK
International Journal of Medical Sciences, 1(2), 101-101 (2004)
Debrup Sengupta et al.
The EMBO journal, 38(16), e99266-e99266 (2019-07-05)
During MHC-I-restricted antigen processing, peptides generated by cytosolic proteasomes are translocated by the transporter associated with antigen processing (TAP) into the endoplasmic reticulum, where they bind to newly synthesized MHC-I molecules. Dendritic cells and other cell types can also generate
S C Roy et al.
International journal of andrology, 31(1), 12-24 (2007-03-16)
The aim of the present study was to determine the effect of L-arginine on nitric oxide (NO*) synthesis, capacitation and protein tyrosine phosphorylation in buffalo spermatozoa. Ejaculated buffalo spermatozoa were capacitated in the absence or presence of heparin, or L-arginine
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.