F9424

Fibroblast Growth Factor Receptor-2α (IIIb)/Fc Chimera human

>90% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder

• Sinonimo/i: FGFR-2α (IIIb)/Fc
• UNSPSC Code: 51111800
• MDL number: MFCD03456786

Proprietà

• biological source: human
• recombinant: expressed in baculovirus infected Sf21 cells
• assay: >90% (SDS-PAGE)
• form: lyophilized powder
• potency: 1-3 ng/mL ED₅₀
• mol wt: monomer calculated mol wt 66 kDa, ~100 kDa by SDS-PAGE (reducing)
• storage condition: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
• impurities: <1 EU/μg Endotoxin level (LAL test)
• UniProt accession no.: P21802
• storage temp.: −20°C
• Gene Information: human ... FGFR2(2263)

Descrizione

General description

FGFR2 (fibroblast growth factor receptor 2) is a member of the tyrosine kinase receptor family called FGFR. This gene is localized to human chromosome 10q26, and is composed of 2 exons.
Its ligand-binding domain in present in its extracellular region, which is composed of three immunoglobulin (Ig)-like domains. It spans the membrane once, and its tyrosine kinase domain is localized to its intracellular region. Alternative splicing of this gene gives rise to two isoforms- epithelial (FGFR2IIIb) and mesenchymal (FGFR2IIIc).

Biochem/physiol Actions

FGFR2 (fibroblast growth factor receptor 2) facilitates tumor growth, invasiveness, metastasis and angiogenesis. It plays an essential role in the development of mammary glands, and its expression is elevated in breast cancers. Studies in North Indian population suggest that polymorphisms in this gene might be linked to susceptibility to breast cancer. It is anti-apoptotic in nature, and suppresses p53-mediated apoptosis, by inducing the elevation of MDM2 levels. Mutations in this gene are linked to mild Crouzon syndrome and craniosynostosis.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Analysis Note

The bioactivity is measured by its ability to inhibit human fibroblast growth factor acidic-dependent proliferation of NR6 cells.

Other Notes

Human recombinant FGFR-2α (IIIb)/Fc is a disulfide-linked homodimeric protein. As a result of glycosylation, it migrates as an approximately 100 kDa protein.