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E4011

ExtrAvidin®−R-Phycoerythrin

buffered aqueous solution

Sinonimo/i:

R-Phycoerythrin

Autenticati per visualizzare i prezzi organizzativi e contrattuali.

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Cambia visualizzazione
Taglio della confezioneSKUDisponibilitàPrezzo
1 mL
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
220,00 €

Informazioni su questo articolo

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00163600
Form:
buffered aqueous solution

220,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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conjugate

phycoerythrin (R-PE) conjugate

Quality Level

200

form

buffered aqueous solution

storage condition

protect from light

extent of labeling

1-4 mol PE per mol ExtrAvidin®

technique(s)

flow cytometry: suitable using human peripheral blood lymphocytes with biotinylated antibody, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human tissues

storage temp.

2-8°C

General description

ExtrAvidin is a chemically modified protein purified from egg whites.
The extravidin-R-phycoerythrin protein can be conjugated with fluorescein-labelled arylacetamide, a κ opioid ligand and biotin-conjugated anti-fluorescein IgG to identify the subpopulations of thymocytes that express κ opioid receptor. This product has shown specificity for rabbit anti-avidin in immunoelectrophoresis analysis.
ExtrAvidin, a uniquely modified avidin reagent, combines the high specific activity and sensitivity of avidin with the low background staining of streptavidin. Avidin consists of four identical subunits, each containing a single biotin binding site.

Immunogen

Thymocytes present in C57BL mice.

Application

The ExtrAvidin-R-Phycoerythrin (PE) conjugate may be used in conjunction with biotinylated reagents in the avidin/biotin labeling system in flow cytometry.
ExtrAvidin®-R-Phycoerythrin has been used
  • In flow cytometric analysis to confirm membrane expression of ncamp-1 (cationic oligodeoxynucleotide binding protein).
  • In indirect immunofluorescence.
  • In Western blotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Questo articolo
E2886E2636E2761
ExtrAvidin®−R-Phycoerythrin buffered aqueous solution

Sigma-Aldrich

E4011

ExtrAvidin®−R-Phycoerythrin

ExtrAvidin®−Peroxidase buffered aqueous solution

Sigma-Aldrich

E2886

ExtrAvidin®−Peroxidase

ExtrAvidin® –Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

E2636

ExtrAvidin® –Alkaline Phosphatase

ExtrAvidin®−FITC buffered aqueous solution

Sigma-Aldrich

E2761

ExtrAvidin®−FITC

technique(s)

flow cytometry: suitable using human peripheral blood lymphocytes with biotinylated antibody, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human tissues

technique(s)

direct ELISA: 1:2,000, dot blot: 1:4,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues, indirect immunofluorescence: 1:200

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage condition

protect from light

storage condition

-

storage condition

-

storage condition

-

conjugate

phycoerythrin (R-PE) conjugate

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

FITC conjugate

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Classe di stoccaggio

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
0000406692
0000372608
0000221256
0000307910
0000202680

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Contenuto correlato

Data Sheet - E4011

Instructions

D M Lawrence et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(4), 1062-1066 (1995-02-14)
A method to visualize the kappa opioid receptor is described that uses a high-affinity fluorescein-conjugated opioid ligand and indirect immunofluorescence with the phycoerythrin fluorophore to amplify the signal. The mouse thymoma cell line R1E/TL8x.1.G1.OUAr.1 (R1EGO), which expresses the kappa 1
T A Ignatowski et al.
The Journal of pharmacology and experimental therapeutics, 284(1), 298-306 (1998-01-22)
Recent studies have shown kappa opioid receptor labeling on the R1EGO thymoma cell line by indirect immunofluorescence and flow cytometric analysis. The present study used a fluorescein-labeled arylacetamide (FITC-AA), a kappa opioid ligand, in conjunction with biotin-conjugated anti-fluorescein IgG and
Molecular characterization of a novel pattern recognition protein from nonspecific cytotoxic cells: sequence analysis, phylogenetic comparisons and anti-microbial activity of a recombinant homologue
Evans DL, et al.
Developmental and Comparative Immunology, 29(12), 1049-1064 (2005)
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Numero articolo commerciale globale

SKUGTIN
E4011-1ML04061833603505

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