The B9059 BACE-1 product included in the kit, serves as a positive control. The Technical Bulletin notes that the concentration of this item is approximate 3 units/microliter. This material has not been tested for purity. Therefore, a molar concentration cannot be provided.
CS0010
β-Secretase (BACE1) Activity Detection Kit (Fluorescent)
1 kit sufficient for 250 reactions
Sinonimo/i:
BACE1 Activity Detection Kit (Fluorescent)
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Informazioni su questo articolo
NACRES:
NA.84
UNSPSC Code:
12161503
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Permettici di aiutartiusage
kit sufficient for 250 reactions
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
human ... BACE1(23621)
Categorie correlate
1 of 4
Questo articolo | |||
|---|---|---|---|
| Gene Information human ... BACE1(23621) | Gene Information human ... BACE1(23621) | Gene Information human ... BACE1(23621) | Gene Information human ... BACE1(23621) |
| usage kit sufficient for 250 reactions | usage - | usage - | usage - |
| Quality Level 300 | Quality Level 200 | Quality Level - | Quality Level 100 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. - |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
Application
The kit provides all the reagents required for an efficient detection of BACE1 activity. It contains an enzyme to be used for screening for potential BACE1 inhibitors. The assay is based on the fluorescence resonance energy transfer (FRET) method in which the fluorescence signal enhancement is observed after substrate cleavage by BACE1.
Biochem/physiol Actions
BACE1 is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer′s. BACE1 is a target for inhibitor drug discovery.
Solo come componenti del kit
N° Catalogo
Descrizione
- Fluorescent Assay Buffer 50 mL
- Stop Solution 15 mL
- Substrate (MOCA-SEV-NL-DAEFR-DNP-RR) 500 μL
- Assay Standard 140 μL
- BACE1 (β−Secretase) 300 units 100 μL
Classe di stoccaggio
10 - Combustible liquids
wgk
WGK 3
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Terrusnolides A-D (1-4), four butenolides were isolated from an endophytic Aspergillus from Tripterygium wilfordii. The structures of 1-4 were established by comprehensive spectroscopic analyses and electronic circular dichroism (ECD) calculation. It is interesting that 1 was a butenolide derived by
Lucas J Gutierrez et al.
Journal of biomolecular structure & dynamics, 35(2), 413-426 (2016-01-28)
We report here two new small-size peptides acting as modulators of the β-site APP cleaving enzyme 1 (BACE1) exosite. Ac-YPYFDPL-NH2 and Ac-YPYDIPL-NH2 displayed a moderate but significant inhibitory effect on BACE1. These peptides were obtained from a molecular modeling study.
Piyoosh Sharma et al.
ACS chemical neuroscience, 10(10), 4361-4384 (2019-09-07)
Multitargeted hybrids of N-benzylpiperidine and substituted 5-phenyl-1,3,4-oxadiazoles were designed, synthesized, and evaluated against Alzheimer's disease (AD). Tested compounds exhibited moderate to excellent inhibition against human acetylcholinesterase (hAChE), butyrylcholinesterase (hBChE), and beta-secretase-1 (hBACE-1). The potential leads 6g and 10f exhibited balanced
β-secretases as a target for the treatment of Alzheimer's.
Citron, M.
Journal of Neuroscience Research, 70, 373-379 (2003)
Judite R M Coimbra et al.
Biomolecules, 10(4) (2020-04-05)
The treatment options for a patient diagnosed with Alzheimer's disease (AD) are currently limited. The cerebral accumulation of amyloid-β (Aβ) is a critical molecular event in the pathogenesis of AD. When the amyloidogenic β-secretase (BACE1) is inhibited, the production of
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what is the concentration of BACE1 enzyme in micromolar?
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