C4879

α-Chymotrypsinogen A from bovine pancreas

essentially salt-free, lyophilized powder

• Sinonimo/i: chymotrypsin A zymogen
• Numero CAS: 9035-75-0
• Numero CE: 232-905-3
• Numero MDL: MFCD00130483
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Origine biologica: bovine pancreas
• Livello qualitativo: 200
• Tipo: Type II
• Forma fisica: essentially salt-free, lyophilized powder
• Attività specifica: ≥40 units/mg solid
• PM: 25,656 Da by calculation
• Purificato mediante: 6× crystallization
• Solubilità: 1 mM HCl: soluble 10 mg/mL, clear, colorless
• N° accesso UniProt: P00767
• Attività estranea: α-chymotrypsin ≤1 U/mg (prior to activation by trypsin)
• Temperatura di conservazione: −20°C
• Informazioni sul gene: cow ... CTRB1(618826)

Descrizione

Descrizione generale

Chymotrypsinogen from bovine pancreas is a zymogen containing 5 disulfide bridges. It has an isoelectric pH of 8.97.

Applicazioni

α-Chymotrypsinogen A from bovine pancreas has been used as model protein crystallization reproducibility studies. It has also been used in the hydrolysis of α-gliadins prior to mass spectroscopy studies.

The enzyme from Sigma has been used in the non-invasive determination of solid-state protein conformation using near infrared (NIR) spectroscopy. It has been used to study the partitioning of protein in polymer/polymer aqueous two-phase systems. The enzyme has also been used for self-interaction chromatography applications, to test the rapid measurement of protein osmotic second virial coefficients. In this technique, the protein is immobilized on chromatographic particles and its retention is measured using isocratic elution.

Azioni biochim/fisiol

Chymotrypsinogen A requires limited proteolysis for its activation. Chymotrypsinogen A may be activated by trypsin and chymotrypsin (autolytic activation) to form m α, β, γ, δ and π chymotrypsin (depending upon the conditions of activation). Chymotrypsin is a protease that will preferentially cleave peptides on the carboxyl side of aromatic amino acids including tryptophan, tyrosine, and phenylalanine. It will also hydrolyze peptides on the carboxyl side of leucine, methionine, and alanine.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the peptide bond.

Definizione di unità

After activation to Chymotrypsin, one unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Altre note

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)