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C3888

Sigma-Aldrich

Carboxypeptidase Y from baker′s yeast (S. cerevisiae)

lyophilized powder, ≥50 units/mg protein

Sinonimo/i:

Peptidyl-L-amino acid Hydrolase, Serine Carboxypeptidase

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1 MG
162,00 €
5 MG
551,00 €

162,00 €


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1 MG
162,00 €
5 MG
551,00 €

About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

162,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Grado

Proteomics Grade

Stato

lyophilized powder

Attività specifica

≥50 units/mg protein

PM

61 kDa

Composizione

Protein, ≥75% E1%/280

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

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Categorie correlate

Applicazioni

Carboxypeptidase Y has a broad specificity and is stable in urea. Hence, this enzyme is not like other carboxypeptidases and can be used for sequence analysis. Due to its amidase action, this enzyme might be applied to the sequence analysis of peptides having amidated COOH-terminal groups such as oxytocin and vasopressin.[1]

Azioni biochim/fisiol

The glycoprotein has a molecular weight of about 61,000, has a nitrogen content of 12.74%. It is a single polypeptide chain of 442 residues with 16 residues of glucosamine in the carbohydrate moiety. Lysine is at the NH2 terminus and -Asp-Ser-Thr-Leu is the COOH-terminal sequence. The principal action of the enzyme is to remove COOH-terminal residues from polypeptide chains.[1] It is used as a vacuolar marker enzyme for studies on protein transport and localization.[2]

Confezionamento

Package size based on protein content

Definizione di unità

One unit will hydrolyze 1.0 μmole of N-CBZ-Phe-Ala to N-CBZ-L-phenylalanine and L-alanine per min at pH 6.75 at 25°C, based on EM/230 = 191.5.

Stato fisico

Lyophilized powder containing citric acid.

Risultati analitici

amidase and esterase activities may be present

Pittogrammi

Health hazardExclamation mark

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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The activity of serine carboxypeptidases is dependent on a catalytic triad, an oxyanion hole, and a binding site equivalent to those found in the serine endopeptidases. The action of carboxypeptidase Y on substrates containing amino acids, alcohols, and amines as
E van Tuinen et al.
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