A6362
Alpha-lytic protease
Sinonimo/i:
Alpha-lytic endopeptidase, alphaLP
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
Origine biologica
bacterial
Forma fisica
liquid
Attività specifica
≥0.0005 U/mg
PM
19,860 Da
pH ottimale
5.0(storage)
7.5(activity)
pI
9.69
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Descrizione generale
Alpha-lytic protease (aLP) is an alternative specificity protease for proteomics applications. This protease cleaves after T, A, S, and V residues. It generates peptides of similar average length as trypsin.
aLP was first isolated from the myxobacterium Lysobacter enzymogenes. The pro-form of aLP is 397 amino acids long. In its mature form, aLP is 198 amino acids long. Its tertiary structural core resembles those of pancreatic serine proteases.
Crystal structure studies of aLP have been reported. Several studies are available on the active site and catalytic mechanism of aLP. The role of the pro-region in the activation, secretion and folding of aLP has been studied.
The activity of aLP in the presence of various solution components is as follows:
aLP was first isolated from the myxobacterium Lysobacter enzymogenes. The pro-form of aLP is 397 amino acids long. In its mature form, aLP is 198 amino acids long. Its tertiary structural core resembles those of pancreatic serine proteases.
Crystal structure studies of aLP have been reported. Several studies are available on the active site and catalytic mechanism of aLP. The role of the pro-region in the activation, secretion and folding of aLP has been studied.
The activity of aLP in the presence of various solution components is as follows:
- 0.1% sodium deoxycholate: ~1.75-fold enhanced activity
- 1.0% sodium deoxycholate: ~60% activity
- 0.1% SDS: ~50% activity
- 1.0% SDS: ~40% activity
- 1 M guanidine HCl: ~20% activity
- 4 M guanidine HCl: ~1% activity (essentially inactivated)
Definizione di unità
One unit will produce one mmole of p-nitroaniline per minute from N-succinyl-Ala-Ala-Ala-PNA at 25 °C at pH 7.5
Stato fisico
Supplied as a solution in 10 mM sodium acetate buffer, pH 5.0.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Molecular structure of the alpha-lytic protease from Myxobacter 495 at 2.8 Angstroms resolution.
G D Brayer et al.
Journal of molecular biology, 131(4), 743-775 (1979-07-15)
Hangping Yao et al.
Cell, 183(3), 730-738 (2020-09-28)
SARS-CoV-2 is an enveloped virus responsible for the COVID-19 pandemic. Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled. Here we report the molecular assembly of the authentic
M Fujinaga et al.
Journal of molecular biology, 184(3), 479-502 (1985-08-05)
The structure of alpha-lytic protease, a serine protease produced by the bacterium Lysobacter enzymogenes, has been refined at 1.7 A resolution. The conventional R-factor is 0.131 for the 14,996 reflections between 8 and 1.7 A resolution with I greater than
C A Bauer et al.
European journal of biochemistry, 120(2), 289-294 (1981-11-01)
The active site of alpha-lytic protease has been studied with a number of synthetic peptides and compared with similar data published for elastase. The kinetic data indicate that the active site of alpha-lytic protease extends over at least six subsites
Site specific N- and O-glycosylation mapping of the spike proteins of SARS-CoV-2 variants of concern.
Shajahan, et al.
Scientific Reports, 13, 10053-10053 (2023)
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.