A6154

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Goat Anti-Rabbit IgG (whole molecule)–HRP
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162788
• Conjugate: peroxidase conjugate
• Clone: polyclonal
• Application: ELISA (d)
• Citations: 782

Proprietà

• biological source: goat
• Quality Segment: 200
• conjugate: peroxidase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: rabbit
• technique(s): direct ELISA: 1:10,000
• storage temp.: −20°C
• target post-translational modification: unmodified

Descrizione

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody is immunospecific for rabbit IgG by immunoelectrophoresis against normal serum and rabbit IgG, prior to conjugation.

Immunogen

purified rabbit IgG

Application

C33A cell lysates were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Protein aliquots from colonic mucosal extracts (μg) were anlayzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody at a 1:5000 dilution in TBSt for 1 hr at room temperature.

Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.

Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody has been used for western blot (1:6,000), and ELISA assays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Pittogrammi: GHS07,GHS09
• Parola di avvertenza: Warning
• Codici di pericolo: H315,H317,H319,H411
• Avvertenze di sicurezza: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Classe di stoccaggio: 12 - Non Combustible Liquids
• Che succede?: WGK 3