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A2404

Sigma-Aldrich

Alcohol Oxidase solution from Pichia pastoris

buffered aqueous solution, 10-40 units/mg protein (biuret)

Sinonimo/i:

Alcohol:oxygen oxidoreductase

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250 UNITS
68,30 €
1000 UNITS
195,00 €

68,30 €

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250 UNITS
68,30 €
1000 UNITS
195,00 €

About This Item

Numero CAS:
9073-63-6
Classificazione EC (Enzyme Commission):
1.1.3.13 (BRENDA, IUBMB)
Numero MDL:
MFCD00130452
Codice UNSPSC:
12352204
NACRES:
NA.54

68,30 €

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Stato

buffered aqueous solution

Attività specifica

10-40 units/mg protein (biuret)

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

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Descrizione generale

Alcohol oxidase (AOX) is a homo-octamer, which has a molecular weight of 600-kDa. It has eight flavin adenine dinucleotide (FAD) cofactors. It belongs to the glucose-methanol-choline (GMC) oxidoreductase family.[1]

Applicazioni

Alcohol Oxidase may be used to study protein translocation into peroxisomes. Product A2404 is from Pichia pastoris. It has been used for the bacterial expression and immunological verification of Hv-p68 cDNA clones [2].
Alcohol Oxidase solution from Pichia pastoris has been used in cotton pectin methylesterase (PME) enzyme assay.[3] It has also been used to immobilize onto polyaniline (PANI) film as a visual biosensor in dip-stick format for the determination of ethanol in fermented beverage samples.[4]

Azioni biochim/fisiol

Alcohol oxidase catalyzes the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It has the highest affinity for methanol. Alcohol oxidase is involved in the metabolism of methanol, which is important for yeasts that utilize methanol as their sole energy source. Alcohol oxidase is primarily found in the peroxisome but may be present in the cytoplasm as well.

Definizione di unità

One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.

Stato fisico

Solution in 30% sucrose with 0.1 M phosphate buffer at pH 8.0

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000390827
0000299797
0000373621
0000310609
0000390827

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Pectin methylesterase and pectin remodelling differ in the fibre walls of two gossypium species with very different fibre properties
Liu Q, et al.
PLoS ONE, 8(6), e65131-e65131 (2013)
Xuan Wang et al.
Journal of experimental botany, 71(9), 2629-2640 (2020-02-02)
De-esterification of homogalacturonan (HG) is thought to stiffen pectin gels and primary cell walls by increasing calcium cross-linking between HG chains. Contrary to this idea, recent studies found that HG de-esterification correlated with reduced stiffness of living tissues, measured by
A I Soldevila et al.
The Journal of biological chemistry, 276(7), 4652-4661 (2000-11-01)
We have cloned and sequenced a novel alcohol oxidase (Hv-p68) from the filamentous fungus Helminthosporium (Cochliobolus) victoriae that copurifies with mycoviral double-stranded RNAs. Sequence analysis revealed that Hv-p68 belongs to the large family of FAD-dependent glucose methanol choline oxidoreductases and
Structure of alcohol oxidase from Pichia pastoris by cryo-electron microscopy
Vonck J, et al.
PLoS ONE, 11(7), e0159476-e0159476 (2016)
Christina Dorado et al.
Biology, 10(12) (2021-12-25)
Florida citrus production has declined 75% due to Huanglongbing (HLB), a disease caused by the pathogenic bacterium Candidatus Liberibacter asiaticus (CLas). Methods to combat CLas are costly and only partially effective. The cross-compatible species Poncirus trifoliata and some of its
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Protocolli

Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)

To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.

Domande

  1. What is the concentration of AOX in mg in 1 unit

    1 risposta

    1. The protein concentration in mg/mL and enzyme activity in unit/mg are lot-specific and reported on the product Certificate of Analysis.

      Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/a2404#product-documentation

      Utile?

Recensioni

Nessuna valutazione

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