A1047

Anti-IgG di topo (specifico per catena γ)−fosfatasi alcalina

affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Goat Anti-Mouse IgG (γ-chain specific)−AP
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162632
• Conjugate: alkaline phosphatase conjugate
• Clone: polyclonal
• Application: ELISA (d), WB
• Citations: 9

Proprietà

• biological source: goat
• Quality Segment: 200
• recombinant: expressed in goat
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: mouse
• technique(s): direct ELISA: 1:7,000-1:21,000, western blot: suitable
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Descrizione

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibody associates with mouse IgG (gamma-chain specific).

Immunogen

purified mouse IgG

Application

Anticorpi anti Trypanosoma cruzi sono stati rilevati nei sieri di topi infettati grazie al test ELISA con un anticorpo di capra anti IgG di topo coniugato con fosfatasi alcalina specifico per catena. Le piastre sono state rivestite con antigene di T. cruzi e bloccate con BSA/PBS all′1% prima dell′incubazione con l′anticorpo coniugato.[1]

For detection of IgG antibodies in mouse sera and saliva following S. gordonii infection, ELISA assays were performed using anti-mouse IgG alkaline phosphatase antibody at 1:20000. This antibody did not detect IgA antibodies.

Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibodies can be used for western blot assays,.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Classe di stoccaggio: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable