93773

TWEEN^® 20

viscosity 250-450 mPa.s (25 °C)

• Sinonimo/i: Monolaurato di polietilenglicole sorbitano, Monolaurato di poliossietilene sorbitano
• Numero CAS: 9005-64-5
• Numero MDL: MFCD00165986
• Codice UNSPSC: 12161900
• NACRES: NA.21

Proprietà

• Descrizione: non-ionic
• Stato: viscous liquid
• PM: ~1228
• tecniche: DNA extraction: suitable; RNA extraction: suitable; cell culture | mammalian: suitable; protein purification: suitable
• Indice di rifrazione: n20/D 1.468 (lit.); n20/D 1.471
• Viscosità: 250-450 mPa.s(25 °C)
• HLB: 16.7±1.0
• Densità: 1.11 g/mL at 20 °C; 1.095 g/mL at 25 °C (lit.)
• Stringa SMILE: CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
• InChI: 1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3; HMFKFHLTUCJZJO-UHFFFAOYSA-N

Descrizione

Descrizione generale

Tween^®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.[1]

Applicazioni

Detergente non ionico

Tween^® 20 is widely used in several biochemical applications. It has been used:

• as an emulsifying agent for the preparation of stable oil-in-water emulsions[2]
• in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)[3]
• as a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%[4][5][6]
• for lysing mammalian cells at a concentration of 0.005 to 0.5%
• for rehydrating tissue before in situ hybridization[7]

Azioni biochim/fisiol

Tween^® 20, functioning as a detergent, potentially operates by binding to the hydrophobic components of water-insoluble substances, thereby making them hydrophilic.

Caratteristiche e vantaggi

• Suitable for protein purification and DNA and RNA extraction
• Suitable for mammalian cell culture
• High purity product for research applications

Avvertenza

may get turbid on storage, heat to clear

Altre note

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Note legali

TWEEN is a registered trademark of Croda International PLC

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): 527.0 °F - Pensky-Martens closed cup
• Punto d’infiammabilità (°C): 275 °C - Pensky-Martens closed cup
• Dispositivi di protezione individuale: Eyeshields, Gloves