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93337

Sigma-Aldrich

Trizma® acetate

BioUltra, ≥99.0% (NT)

Sinonimo/i:

TRIS acetate salt, Tris(hydroxymethyl)aminomethane acetate salt

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About This Item

Formula condensata:
NH2C(CH2OH)3 · CH3COOH
Numero CAS:
6850-28-8
Peso molecolare:
181.19
Beilstein:
3702918
Numero CE:
229-939-6
Numero MDL:
MFCD00038951
Codice UNSPSC:
12161700
ID PubChem:
57653535
NACRES:
NA.25

Nome Commerciale

BioUltra

Saggio

≥99.0% (NT)

Forma fisica

crystalline powder

Impurezze

insoluble matter, passes filter test

Residuo alla calcinazione

≤0.2%

Perdita

≤0.2% loss on drying, 20 °C (HV)

pH

6.0-7.0 (25 °C, 0.5 M in H2O)

Solubilità

H2O: 0.5 M at 20 °C, clear, colorless

Anioni in tracce

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

Cationi in tracce

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.5 M in H2O

Assorbanza UV

λ: 260 nm Amax: 0.07
λ: 280 nm Amax: 0.05

Stringa SMILE

CC(O)=O.NC(CO)(CO)CO

InChI

1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4)
PIEPQKCYPFFYMG-UHFFFAOYSA-N

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Applicazioni

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma acetate is used to make Tris acetic acid buffers that are used as diluents for various assays and as an electrophoresis running buffers.

Altre note

Recommended buffer for maximum sensitivity of ATP assays with firefly luciferase; Assay of glutamate binding

Note legali

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Monica Cubillos-Rojas et al.
Methods in molecular biology (Clifton, N.J.), 869, 205-213 (2012-05-16)
Polyacrylamide gel electrophoresis (PAGE) is one of the most powerful tools used for protein analysis. We describe the use of Tris-acetate buffer and 3-15% polyacrylamide gradient gels to simultaneously separate proteins in the mass range of 10-500 kDa. We show
M Ito et al.
Life sciences, 38(12), 1089-1096 (1986-03-24)
[3H]L-glutamic acid binding to microfuge tubes and glass was investigated in four buffers. Background binding to these materials was negligible, but was increased by centrifugation or suction in Tris-HCl and Tris-citrate buffer. This binding was much less or eliminated when
Monica Cubillos-Rojas et al.
Electrophoresis, 31(8), 1318-1321 (2010-03-24)
To separate and analyze giant and small proteins in the same electrophoresis gel, we have used a 3-15% polyacrylamide gradient gel containing 2.6% of the crosslinker bisacrylamide and 0.2 M of Tris-acetate buffer (pH 7.0). Samples were prepared in a
Choice of buffer anion for the assay of adenosine 5'-triphosphate using firefly luciferase.
W W Nichols et al.
Analytical biochemistry, 114(2), 396-397 (1981-07-01)
Chi-Lin Li et al.
The Analyst, 137(22), 5222-5228 (2012-10-04)
Oligonucleotide (T30695) modified gold nanoparticles (T30695-Au NPs) have been prepared and employed for quantification of lead ions (Pb(2+)) in blood. The detection of Pb(2+) ions is through the formation of Au-Pb alloys and oligonucleotide-Pb(2+) complexes that catalyze the H(2)O(2)-mediated oxidation
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