62310

Lipase from Rhizopus niveus

powder (fine), ≥1.5 U/mg

• Sinonimo/i: Triacylglycerol acylhydrolase, Triacylglycerol lipase
• Numero CAS: 9001-62-1
• Classificazione EC (Enzyme Commission): 3.1.1.3 (BRENDA, IUBMB)
• Numero CE: 232-619-9
• Numero MDL: MFCD00131509
• Codice UNSPSC: 12352204
• NACRES: NA.26

Proprietà

• Origine biologica: fungus (Rhizopus niveus)
• Livello qualitativo: 100
• Forma fisica: powder (fine)
• Attività specifica: ≥1.5 U/mg
• PM: M_r ~83000
• Temperatura di conservazione: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;; QWZUIMCIEOCSJF-CHHCPSLASA-N

Descrizione

Descrizione generale

Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.

Applicazioni

Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.

Azioni biochim/fisiol

Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Definizione di unità

1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314, at pH 8.0 and 40°C as substrate.

Altre note

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function)

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)