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51280

Emina

BioXtra, from Porcine, ≥96.0% (HPLC)

Sinonimo/i:

Cloro(protoporfirinato)ferro(III), Cloroemina, Cloroprotoporfirina IX ferro(III), Cloruro di ferriprotoporfirina IX, Emina(cloruro)

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Cambia visualizzazione
A voi/SKUDisponibilitàPrezzo
1 g
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
66,40 €
5 g
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
247,00 €
25 g
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
990,00 €

Informazioni su questo articolo

Formula empirica (notazione di Hill):
C34H32ClFeN4O4
Numero CAS:
16009-13-5
Peso molecolare:
651.94
UNSPSC Code:
12352202
NACRES:
NA.79
PubChem Substance ID:
57651319
EC Number:
240-140-1
Beilstein/REAXYS Number:
5229914
MDL number:
MFCD00010726
Form:
powder or crystals
Assay:
≥96.0% (HPLC)
Biological source:
Porcine

66,40 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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biological source

Porcine

Quality Segment

200

product line

BioXtra

assay

≥96.0% (HPLC)

form

powder or crystals

technique(s)

tissue culture: suitable, transfection: suitable

storage temp.

2-8°C

SMILES string

Cc1c(CCC(O)=O)c2cc3c(CCC(O)=O)c(C)c4cc5nc(cc6c(C)c(C=C)c(cc1n2)n6[Fe](Cl)n34)c(C=C)c5C

InChI

1S/C34H34N4O4.ClH.Fe/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);1H;/q;;+3/p-3/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;;

InChI key

BTIJJDXEELBZFS-HXFTUNQESA-K

Application

L′emina è usata per le seguenti applicazioni:
  • Per studiare i ceppi batterici e le condizioni di crescita[1]
  • Nei saggi sulle colonie a breve termine (il terreno di coltura è costituito da emina insieme ad altri componenti)[2]
  • Trasfezioni cellulari[3]
Può essere usata nel saggio sulla formazione di β-ematina per analizzare l′attività inibitoria dei composti della Malaria Box di MMV (Medicines for Malaria Venture) nei confronti della formazione di β-ematina.[4]

Biochem/physiol Actions

L′emina, versione ossidata dell′eme, è un gruppo prostetico che contiene ferro per un gruppo di proteine diverse. L′eme libero, che può essere rilasciato dall′emoglobina in caso di emolisi, è un proinfiammatorio e contribuisce alle specie reattive dell′ossigeno derivate dal ferro. I livelli di eme libero possono essere sovraregolati in diverse condizioni patologiche e possono avere un ruolo in varie condizioni infiammatorie inclusi i disturbi vascolari, l′insufficienza renale e i disturbi immunomediati.

L′emina può essere usata per studiare l′espressione e la repressione dell′eme ossigenasi 1 (HO-1) oltre che la sua attività. L′ematina è il “fattore X" necessario per la proliferazione dell′Haemophilus influenzae.

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Questo articolo
H90393741MBD0052
Emina BioXtra, from Porcine, ≥96.0% (HPLC)

Sigma-Aldrich

51280

Emina

Emina from bovine, ≥90%

Sigma-Aldrich

H9039

Emina

Hemin Chloride Iron-containing compound of porcine origin that can induce hydrogen peroxide-dependent lipid peroxidation. Reported to increase the number and size of myotubes and the frequency of myotube contractions.

Millipore

3741

Hemin Chloride

Hemin solution Ready-made microbial growth medium supplement, 0.5 g/L

Sigma-Aldrich

MBD0052

Hemin solution

biological source

Porcine

biological source

bovine

biological source

-

biological source

-

assay

≥96.0% (HPLC)

assay

≥90%

assay

-

assay

-

technique(s)

tissue culture: suitable, transfection: suitable

technique(s)

toxicology assay: suitable

technique(s)

-

technique(s)

-

form

powder or crystals

form

powder

form

solid

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

15-25°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

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Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number

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Domande

1–8 di 8 domande  

  1. Can 51280 be used to induce the erythroid differentiation of the K562 cells? If yes, what would it need to be dissolved in to add be added to cell culture?

    1 risposta

    1. This product is not tested for any specific application. However, the material has been used successfully by other researchers to induce erythroid differentiation and hemoglobinization of K562 cells. Please see the link below to review one such publication:
      https://pmc.ncbi.nlm.nih.gov/articles/PMC4665220/

      For culture use, this compound is typically prepared in DMSO at a concentration of 1 mM. The material is also soluble in 1.4 M ammonium hydroxide at 24 mg/mL at ambient temperature or 50 mg/ml when heated to 95°C for 5 minutes. Additionally, stock solutions of 0.25% hemin in 50% triethanolamine have been reported. Published methods suggest rates of 40 - 60 µM hemin for a treatment period of 1-5 days. Filter-sterilized stock solutions may be stored refrigerated, protected from light.

      Utile?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Utile?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Utile?

  4. What is the solubility of Hemin, product catalog # 51280, CAS 16009-13-5?

    1 risposta

    1. The product with code 51280 can be dissolved in DMSO at a concentration of 1 mM. It is also soluble in 1.4 M ammonium hydroxide at a concentration of 50 mg/ml, and this can be achieved by heating the solution at 95°C for 5 minutes, resulting in a dark brown solution. Additionally, a stock solution of 0.25% hemin in 50% triethanolamine can be prepared. CAS number 16009-13-5, which corresponds to product 51280, is soluble in 1.4 M NaOH at a concentration of 25 mg/mL, and it is practically insoluble in water.

      Utile?

  5. How can hemin be dissolved for cell culture experiments in macrophages? Is it necessary to couple it to serum/BSA?

    1 risposta

    1. This product has not been tested for use in cell culture. However, a previously deleted product, H1652, has been tested. The H1652 was dissolved at 10 mg/mL in 1 M NaOH and added then added to the culture medium at 7 mg/L. This information would need to be validated for product 51280.

      Utile?

  6. How stable are stock solutions of hemin (dissolved in 1N NaOH and diluted to 1 mM with dH2O)? Can they be stored at 4C or -20C?

    1 risposta

    1. The solution stability has not been determined.

      Utile?

  7. What is the solubility of Product 51280, Hemin?

    1 risposta

    1. Hemin can be dissolved in DMSO at 1 mM. This product should be soluble in 1.4 M ammonium hydroxide (50 mg/ml) with 5 minutes of heating at 95 °C, yielding a dark brown solution.  A stock solution of 0.25% hemin in 50% triethanolamine can be made.

      Utile?

  8. What is the Department of Transportation shipping information for this product?

    1 risposta

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Utile?

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