PC205L
Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb
lyophilized, Calbiochem®
Sinonimo/i:
Anti-CGRP
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About This Item
Codice UNSPSC:
12352203
NACRES:
NA.43
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
serum
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Forma fisica
lyophilized
contiene
≤0.1% sodium azide as preservative
Reattività contro le specie
rat, mouse
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
Isotipo
IgG
Condizioni di spedizione
ambient
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
mouse ... Calca(12310)
Descrizione generale
Anti-CGRP, rabbit polyclonal, recognizes the α-CGRP in rat amygdala and spinal cord. It is validated for immunofluorescence, & for use with frozen, paraffin, and free-floating sections.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-CGRP protein.
Recognizes the α-calcitonin gene related peptide in rat amygdala and spinal cord.
Immunogeno
rat α-calcitonin gene related peptide (α-CGRP)
Applicazioni
Free Floating Sections (see application references)
Frozen Sections (1:2000-1:4000, ABC detection)
Immunofluorescence (1:100-1:200, see application references)
Paraffin Sections (see application references; heat pre-treatment required; see comments)
Attenzione
Toxicity: Standard Handling (A)
Ricostituzione
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.
Risultati analitici
Positive Control
Rat amygdala or spinal cord
Rat amygdala or spinal cord
Altre note
Antibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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