MABE60

Anti-BRG1 Antibody, clone 3G4

clone 3G4, from rat

• Sinonimo/i: Transcription activator BRG1, ATP-dependent helicase SMARCA4, BAF190A, BRG1-associated factor 190A, hSNF2b, Mitotic growth and transcription activator, Protein brahma homolog 1, Protein BRG-1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rat
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 3G4, monoclonal
• Reattività contro le specie: human, mouse
• tecniche: immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_001122316
• N° accesso UniProt: P51532
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SMARCA4(6597)

Descrizione

Descrizione generale

Transcription activator BRG1 (UniProt P51532; also known as ATP-dependent helicase SMARCA4, BAF190A, BRG1-associated factor 190A, hSNF2b, Mitotic growth and transcription activator, Protein brahma homolog 1, Protein BRG-1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4) is encoded by the SMARCA4 (also known as BAF190A, BRG1, MRD16, RTPS2, SNF2B, SNF2L4) gene (Gene ID 6597) in human. The SWI/SNF-like BRG1/BRM-associated factor (BAF) chromatin-remodeling complexes drive conformational changes of nucleosomes in an ATP-dependent manner and play important roles in cell-cycle progression, DNA repair, and development. The SWI/SNF complexes contain 10-12 interchangeable subunits, with either BRG1/SMARCA4 or BRM/SMARCA2 as the ATPase subunit. Brg1 is also shown to maintain Polycomb-mediated repression of non-mesodermal developmental regulators. Brg1-knockout leads to disruption of murine ESC cardiomyocyte differentiation and dysregulation of lineage-specific gene expression during mesoderm induction.

Immunogeno

GST-tagged recombinant protein corresponding to human BRG1.

Applicazioni

Anti-BRG1 Antibody, clone 3G4 is a rat monoclonal Antibody for detection of BRG1, also known as BRG1-associated factor 190A, SNF2-like 4, and SNF2-BETA, and has been validated in Immunocytochemistry and Western Blotting applications.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Transcription Factors

Western Blotting Analysis: A representative lot detected BRG1 (SMARCA4) in THP-1 human leukemia monocytic cell lysates (Cruickshank, V.A., et al. (2015). PLoS One. 10(11):e0142806).

Immunocytochemistry Analysis: A representative lot detected nuclear euchromatin BRG1 immunoreactivity in C2C12 murine myoblasts (Ohkawa, Y., et al. (2009). Hybridoma (Larchmt). 28(6):463-466).

Qualità

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.25 µg/mL of this antibody detected BRG1 in 10 µg of HeLa cell lysate.

Descrizione del bersaglio

~185 kDa observed

Stato fisico

Format: Purified

Protein G Purified

Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
HeLa cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable