06-1130-I

Anti-RNF168 Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: E3 ubiquitin-protein ligase RNF168, hRNF168, RING finger protein 168
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• tecniche: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• N° accesso NCBI: NP_689830
• N° accesso UniProt: Q8IYW5
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... RNF168(165918)

Descrizione

Descrizione generale

E3 ubiquitin-protein ligase RNF168 (UniProt Q8IYW5; also known as hRNF168, RING finger protein 168) is encoded by the RNF168 gene (Gene ID 165918) in human. Ubiquitylation/Ubiquitination is an essential early signal in the DNA damage repair process. Multiple E3 ligases, including RNF2 (RING2), RNF8 and RNF168, mediate the addition of K63-linked polyubiquitin chains to histone 2A (H2A) and H2AX, which in turn recruits DNA damage repair proteins, e.g. RAP80, BRCA1. In addition, SUMOylation and NEDDylation are also involved in the DNA damage response. RNF168 is an a dual specificity E3 ligase that mediates both the ubiquitination and NEDDylation of H2A and H2AX. The two types of modifications compete against each other and NEDD8 modification of H2A and H2AX blocks the recruitment of BRCA1 at DNA damage repair sites. RNF168 itself is also subjected to NEDD8 modification and NEDDylation of RNF168 is necessary for its ubiquitin ligase activity. Inhibition of RNF168 NEDDylation impairs its interaction with E2 enzyme Ubc13 (UBE2N). Likewise, downregulating RNF168 NEDDylation by the deNEDDylating enzyme NEDP1, or due to mutations of the NEDD8-conjugating enzyme UBC12, decreases H2A and H2AX ubiquitylation. RNF168 Mutations are linked to RIDDLE (radiosensitivity, immunodeficiency dysmorphic features and learning difficulties) syndrome.

Specificità

This polyclonal antibody detected nuclear RNF168 immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized human B-cell hybridoma SA13.

Immunogeno

GST-tagged full-length human RNF168.

Applicazioni

Anti-RNF168, Cat. No. 06-1130-I, is a highly specific rabbit polyclonal antibody that targets RNF168 and has been tested in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected nuclear RNF168 immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized human B-cell hybridoma SA13.

Immunoprecipitation Analysis: 20 µL from a representative lot immunoprecipitated RNF168 from 500 µg of human B-cell hybridoma SA13 cell lysate.

Qualità

Evaluated by Western Blotting in SA13 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RNF168 in 10 µg of human B-cell hybridoma SA13 cell lysate.

Descrizione del bersaglio

~75 kDa observed. 65.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: 06-1130

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable