G005645

LC/MS/MS of Pesticides in Oranges on Ascentis^® Express C18 after Sample Prep using Supel^™ Que Z-Sep/C18 QuEChERS sorbent

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: sample preparation: Dispersive (QuEChERS); sample/matrix: 10 g of pureed oranges (homogenized with rind); spike at 50 ppb (add 16.75 μL of a custom made pesticide mix, each analyte at 30 μg/mL); extraction tube: Supel^™ QuE Citrate (EN) Tube (55227-U); extraction process: add 10 mL acetonitrile; shake for 1 minute; add contents of a Supel QuE citrate extraction tube (55227-U); shake immediately for 1 minute; centrifuge at 3200 rpm for 5 minutes; clean-up tube: Supel-Q PSA/C18 (55284-U); clean-up process: transfer 0.7 mL of the acetonitrile layer into a Supel QuE Z-Sep/C18 cleanup tube (55284-U); shake for 1 minute; centrifuge at 5000 rpm for 5 minutes; (transfer 0.2 mL of the supernatant into an empty 1.5 mL centrifuge tube; add 0.2 mL of water; centrifuge at 5000 rpm for 2 minutes.); column: Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U); mobile phase: [A] 10 mM ammonium acetate in water; [B] 10 mM ammonium acetate in acetonitrile; gradient: Hold at 30% B for 1 min; 30% to 80% B in 2 min; hold at 80% B for 4 min; hold at 100% B for 3 min; hold at 30% B for 3 min; flow rate: 0.3 mL/min; pressure: 2730 psi (188 bar); column temp.: 30 °C; detector: MS/MS, ESI positive
• suitability: application for HPLC
• application(s): food and beverages

Description

Analysis Note

This demonstrates the application of the Z-Sep/C18 sorbent to the cleanup of fruits in the LC-MS-MS analysis of polar pesticides including organophosphates, carbamates and benzoylureas

Sample pretreatment: 10 g of pureed oranges homogenized with rind; spike at 50 ppb by adding 16.75 μL of a pesticide mix containing each analyte at 30 μg/mL

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Supel is a trademark of Sigma-Aldrich Co. LLC

Analyte

Methomyl, analytical standard
Trichlorfon, PESTANAL^®, analytical standard
Carbendazim, analytical standard
Aldicarb, PESTANAL^®, analytical standard
Parathion-methyl, PESTANAL^®, analytical standard
Methabenzthiazuron, analytical standard
Dibrom^®, PESTANAL^®, analytical standard
Methidathion, analytical standard
Clethodim, analytical standard
Phosmet, PESTANAL^®, analytical standard
Ametryn, PESTANAL^®, analytical standard
Sethoxydim, analytical standard
Anilazine, analytical standard
Fenhexamid, PESTANAL^®, analytical standard
Mecarbam, PESTANAL^®, analytical standard
Oryzalin, analytical standard
Fenoxycarb, PESTANAL^®, analytical standard
Iprobenfos, PESTANAL^®, analytical standard
Quinalphos, analytical standard
Edifenphos, analytical standard
Etrimfos, PESTANAL^®, analytical standard
Fenitrothion, analytical standard
Diazinon, analytical standard
Tolclofos-methyl, PESTANAL^®, analytical standard
Phorate, analytical standard
Chlorpyrifos-methyl, analytical standard
EPN, PESTANAL^®, analytical standard
Terbufos, PESTANAL^®, analytical standard
Ethion, analytical standard
Lufenuron, PESTANAL^®, analytical standard
Spiromesifen, PESTANAL^®, analytical standard
2-Octyl-4-isothiazolin-3-one, PESTANAL^®, analytical standard
Pyraclostrobin, PESTANAL^®, analytical standard
Carbophenothion, analytical standard
Flufenoxuron, PESTANAL^®, analytical standard
Fenpyroximate, PESTANAL^®, analytical standard
See All (36)