ZRB1282

Anti-FUNDC1 Antibody, clone 1K16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1K16, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 17.18 kDa; observed mol wt ~15 kDa
• purified by: using Protein A
• species reactivity: human, rat
• species reactivity (predicted by homology): mouse, monkey, canine, bovine, feline
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_776155.1
• UniProt accession no.: Q8IVP5
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1K16 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects FUN14 domain-containing protein 1 (FUNDC1). It targets an epitope within 20 amino acids from the C-terminal, second cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the second cytoplasmic domain of human FUN14 domain-containing protein 1 (FINDC1).

Application

Quality Control Testing

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FUNDC1 in L6 cell lysate.

Tested applications

Affinity Binding Assay: A representative lot of this antibody bound FUNDC1 with a KD of 1.5 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FUNDC1 in human cerebral cortex tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FUNDC1 in L6 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-FUNDC1, clone 1K16 ZooMAb^®, Cat. No. ZRB1282, is a recombinant Rabbit monoclonal antibody that specifically targets FUNDC1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

FUN14 domain-containing protein 1 (UniProt: Q8IVP5; also known as FUNDC1) is encoded by the FUNDC1 gene (Gene ID: 139341) in human. FUNDC1 is a multi-pass membrane protein with two cytoplasmic domains, three transmembrane domains, and two mitochondrial intermembrane domains. It is widely expressed and is conserved from Drosophila to human. It is localized exclusively in the mitochondria and acts as an activator of hypoxia-induced mitophagy, which contributes to the mitochondrial quality control. It interacts with LC3 family proteins via its LC3 binding motif YXXL (aa 18-21). Knockdown of endogenous FUNDC1 is shown to inhibit hypoxia-induced mitophagy that can be reversed by the expression of wild-type FUNDC1, but not LC3-interaction-deficient FUNDC1 mutants. Mutations leading to defective YXXL motif abolish its interaction with LC3 and subsequent mitophagy. Phosphorylation of tyrosine 18 in the YXXL motif by Src kinase inhibits the activation of mitophagy. Following hypoxia, tyrosine 18 is dephosphorylated that results in increase in interaction with LC3 proteins and triggers mitophagy. FUNDC1 is shown to reduce the levels of TOM20 and VDAC1 in the outer mitochondrial membrane and TIM23 in the inner membrane without affecting the content of other organelle markers. This reduction can be blocked by treating cells with bafilomycin A1. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liu, L., et al. (2012). Nat. Cell Biol. 14; 177-185).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable