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SRP0135

Sigma-Aldrich

G9a human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym(s):

EHMT2, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, KMT1C, Lysine N-methyltransferase 1C

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

74.6 kDa

packaging

pkg of 50 μg

concentration

>0.02 mg/mL

NCBI accession no.

NM_006709

UniProt accession no.

Q96KQ7

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... EHMT2(10919)

General description

Human G9a (euchromatic histone-lysine N-methyltransferase 2) with N-terminal GST tag, MW = 74.6 kDa, expressed in an E. coli expression system. G9a is a member of SET (Su(var)3-9, enhancer-of-zeste and trithorax) domain containing, Su(var)3-9 family of proteins. This domain is evolutionary conserved.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

The encoded protein G9a (euchromatic histone-lysine N-methyltransferase 2) is a methyltransferase that methylates lysine residues of histone H3. Overexpression of G9a is observed in cancer. It is known to participate in cancer cell migration, invasion and metastasis. G9a is an epigenetic regulator and can inhibit the action of tumor suppressor genes, thereby, contributes to carcinogenesis. Thus, G9a is considered to be an oncogenic and metastatic factor. It also affects DNA damage repair mechanism, which might make tumor cells resistant to radiation and chemotherapy. G9a serves as a target for cancer therapy including breast cancer.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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G9a coordinates with the RPA complex to promote DNA damage repair and cell survival
Yang Q, et al.
Proceedings of the National Academy of Sciences of the USA, 114(30), E6054-E6063 (2017)
G9a drives hypoxia-mediated gene repression for breast cancer cell survival and tumorigenesis
Casciello F, et al.
Proceedings of the National Academy of Sciences of the USA, 114(27), 7077-7082 (2017)
H3K27me1 is essential for MMP-9-dependent H3N-terminal tail proteolysis during osteoclastogenesis
Kim K, et al.
Epigenetics & Chromatin, 11(1), 23-23 (2018)
SATB2 suppresses non-small cell lung cancer invasiveness by G9a
Ma Yi-nan, et al.
Clinical and Experimental Medicine, 18(1), 37-44 (2018)
G9a, a multipotent regulator of gene expression
Shankar S, et al.
Epigenetics, 8(1), 16-22 (2013)

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