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SAB4200722

Sigma-Aldrich

Anti-Band 3 antibody, Mouse monoclonal

clone BIII-136, purified from hybridoma cell culture

Synonym(s):

AE 1, Anion exchange protein 1, Anion exchanger 1, Band 3 anion transport protein, Solute carrier family 4 member 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

BIII-136, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1 mg/mL

technique(s)

immunoblotting: 2-4 μg/mL (human erythrocytes ghosts extract)
immunoprecipitation (IP): suitable

isotype

IgG2a

UniProt accession no.

P02730

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SLC4A1(6521)

General description

Anti-Band 3 antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the BIII-136 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with glycophorin B purified from human erythrocytes.

Application

Anti-Band 3 antibody, Mouse monoclonal may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

As a response to an oxidative stress, Band 3 protein forms clusters and clear damaged and aged RBCs from circulation. Enhanced band 3 protein clustering is implicated in RBC disorders such as hemolytic anemia, glucose-6-phosphate dehydrogenase (G6PD) deficiency, malaria and sickle-cell disease.

Physical form

Glycophorin B purified from human erythrocytes
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hanae Shimo et al.
PLoS computational biology, 11(6), e1004210-e1004210 (2015-06-06)
Oxidative stress mediated clustering of membrane protein band 3 plays an essential role in the clearance of damaged and aged red blood cells (RBCs) from the circulation. While a number of previous experimental studies have observed changes in band 3
Changes in band 3 structure as determinants of erythrocyte integrity during storage and survival after transfusion
Bosman G, et al.
Blood Transfusion = Trasfusione del Sangue, 8(Suppl 3), s48-s48 (2010)
Sandrine Genetet et al.
Scientific reports, 7, 46170-46170 (2017-04-08)
Anion Exchanger 1 (AE1) and stomatin are integral proteins of the red blood cell (RBC) membrane. Erythroid and kidney AE1 play a major role in HCO3- and Cl- exchange. Stomatins down-regulate the activity of many channels and transporters. Biochemical studies

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